Altogen Biosystems is a life sciences company dedicated to the development, manufacture, and marketing of cell line-specific transfection reagents for intracellular delivery of biomolecules. Efficient delivery of siRNA and plasmid DNA enabled into broad range of cell types and primary cells by advanced formulation of reagents and peculiar design of protocols. Altogen Biosystems develops polymer-, lipid-, and nanoparticle-based transfection technologies by applying expertise in molecular biology, combinatorial chemistry, and cell biology.

Altogen Custom Services offer custom research services, including generation of stable cell lines, cloning, RNA Interference (RNAi) and gene silencing services, assay development, siRNA library screening, and transfection services. Generation of stably-expressing cancer cell lines and primary cells can be very expensive and time-consuming. Our cell culture scientists can transform the cell line of choice to stably express gene of interest, or shRNA-expressing vector. Standard RNAi service include design and synthesis of siRNA targeting gene of interest, validation of siRNA silencing efficiency, and optimization of siRNA transfection conditions to enable highly efficient gene silencing in your cell line or cultured primary cells.

Transfection is the transient or stable introduction of exogenous molecules and genetic material, RNA or DNA, into cultured cells and is commonly utilized in biological laboratories for studying gene function, modulation of gene expression, biochemical mapping, mutational analysis, and protein production. Scientists use various carrier molecules to enable gene delivery of such molecules as plasmid DNA (pDNA), messenger RNA (mRNA), short interfering RNA (siRNA), microRNA (miRNA), and proteins into cancer cell lines and primary cells. Unfortunately, no single delivery method or transfection reagent can be applied to all types of cells; cellular cytotoxicity and transfection efficiencies vary dramatically depending on the reagent, protocol and cell type being utilized. Altogen Biosystems offers pre-optimized transfection kits for more than 60 cell types.

Nanoparticle-, Lipid-, and Polymer-based ALTOGEN® In Vivo Transfection Reagents enable delivery of functional RNA and DNA molecules in vivo. PEG-Liposome in vivo delivery system reduce innate immune response due to PEG modification, and provides efficient transfection of siRNA, DNA, and proteins in vivo.

Altogen Biosystems presents featured cell line-specific transfection kits:

In Vivo Transfection Reagents | A549 Transfection Reagent | HepG2 Transfection Reagent | MEF Transfection Reagent | Astrocyte Transfection Reagent | Fibroblast Transfection Reagent | MDA-MB Transfection Reagent | HUVEC Transfection Reagent | LNCaP Transfection Reagent | MCF-7 Reagent

New Altogen® transfection products:

DNA and siRNA Electroporation Kit | Cell Cycle Arrest siRNA | Apoptosis-inducing siRNA

Visit Altogen's Transfection Resource: RNAi applications, siRNA transfection, stable and transient transfection, delivery methods, in vitro and in vivo applications, virus-mediated gene delivery, nanoparticle-, liposome-, and biodegradable polymer-based invivo transfection reagents.