Altogen Biosystems is a life sciences company dedicated to the development, manufacture, and marketing of cell line-specific transfection reagents for the intracellular delivery of biomolecules and the efficient delivery of siRNA and plasmid DNA into a broad range of cell types and primary cells by advanced formulation of reagents and protocol design. Altogen Biosystems develops polymer-, lipid-, and nanoparticle-based transfection technologies by applying expertise in molecular biology, combinatorial chemistry, and cell biology.

Altogen Custom Services offers GLP-compliant custom research services, including generation of stable cell lines, cell banking and cryopreservation, pyrosequencing, cloning, RNA interference (RNAi) and gene silencing services, assay development, siRNA library screening, and transfection services. The generation of stable cancer cell lines and primary cells can be very expensive and time-consuming. The company’s cell culture scientists transform cell lines of choice to either stably express genes of interest or shRNA-expressing vectors. Standard RNAi services include design and synthesis of siRNAs that target genes of interest, validation of siRNA silencing efficiency, and optimization of siRNA transfection conditions to enable efficient gene silencing in cell lines or cultured primary cells.

Transfection is the transient or stable introduction of exogenous molecules and genetic material (i.e., RNA or DNA) into cultured cells and is commonly utilized in biological laboratories to study gene function, modulation of gene expression, biochemical mapping, mutational analysis, and protein production. Scientists use various carrier molecules to enable gene delivery of such molecules as plasmid DNA (pDNA), messenger RNA (mRNA), short interfering RNA (siRNA), microRNA (miRNA), and proteins into cancer cell lines and primary cells. Unfortunately, no single delivery method or transfection reagent can be applied to all types of cells; cellular cytotoxicity and transfection efficiencies vary dramatically and depend on the reagent, protocol ,and cell type being utilized. Altogen Biosystems offers pre-optimized transfection kits for more than 60 cell types.

Nanoparticle-, Lipid-, and Polymer-based ALTOGEN® In Vivo Transfection Reagents enable the delivery of functional RNA and DNA molecules in vivo. The PEG-Liposome in vivo delivery system reduces innate immune responses due to PEG modification, and provides efficient transfection of siRNA, DNA, and protein in vivo.

Featured by Science magazine (December 17, 2010): PEG-Liposome siRNA In Vivo Transfection Kit

Altogen Biosystems presents featured cell line-specific transfection kits:

In Vivo Transfection Reagents | A549 Transfection Reagent | HepG2 Transfection Reagent | MEF Transfection Reagent | Astrocyte Transfection Reagent | Fibroblast Transfection Reagent | MDA-MB Transfection Reagent | HUVEC Transfection Reagent | LNCaP Transfection Reagent | MCF-7 Reagent

New Altogen® transfection products and services:

Cell Banking Services: Cell Growth, MCB Generation, and Cryopreservation (GLP-Compliant Quality System)

DNA and siRNA Electroporation Kit | Cell Cycle Arrest siRNA | Apoptosis-inducing siRNA

Visit Altogen's Transfection Resource: RNAi applications, siRNA transfection, stable and transient transfection, delivery methods, in vitro and in vivo applications, virus-mediated gene delivery, nanoparticle-, liposome-, and biodegradable polymer-based invivo transfection reagents.