Pancreas In Vivo Transfection Kit

Catalog #5050 PANCREAS-targeted In Vivo Transfection Reagent - 0.5 mL (10 injections) $495

Catalog #5051 PANCREAS-targeted In Vivo Transfection Reagent - 1.5 mL (30 injections) $995

Pancreas In Vivo Transfection Reagent

siRNA and DNA pancreas in vivo delivery reagent for animal research (mouse, rat)

Modes of administration:

  • Systemic intravenous (i.v.) injection
  • Direct intratumoral (i.t.) injection
  • Intraperitoneal (i.p.) injection

Pancreas-targeted In Vivo Transfection Reagent

  • Biodegradable lipid liposome-based reagent
  • Liposome conjugated complexes are stable in serum (16h)
  • Efficient delivery to the pancreas tissue (via systemic administration) and pancreas tumors (via intratumoral administration)
  • Efficient siRNA, shRNA, microRNA, and plasmid DNA delivery
  • Minimal toxicity
  • Functionally validated in mice
  • Applicable for plasmid DNA/siRNA co-injection
  • Download Pancreas-targeted in vivo transfection protocol: [PDF] [Word]
  • Developed and manufactured by Altogen Biosystems

DATA

In Vivo Pancreas Transfection Reagent Pancreas Pancreatic Tumors

Figure 1. Systemic administration (i.v.) of Pancreas In Vivo Transfection Reagent conjugated with 80 ug of chemically modified siRNA targeting Lamin A/C mRNA or non-silencing control siRNA following the recommended protocol. Tissues (Pancreas, Brain, Muscle, Kidney, Tumor, Heart, Spleen, Lung) were collected and RNA isolated 48 hours after first injection. Samples were analyzed by qRT-PCR for Lamin A/C gene expression levels. Ribosomal RNA levels were used to normalize the Lamin A/C data. Data are means ± SD (n=8).

Pancreas Transfection Kit

Figure 2. Intratumoral administration (i.t.) of Pancreas In Vivo Transfection Reagent conjugated with 80 ug of chemically modified siRNA targeting Lamin A/C mRNA or non-silencing control siRNA following the recommended protocol. Tissues (Pancreas, Brain, Muscle, Kidney, Tumor, Heart, Spleen, Lung) were collected and RNA isolated 48 hours after first injection. Samples were analyzed by qRT-PCR for Lamin A/C gene expression levels. Ribosomal RNA levels were used to normalize the Lamin A/C data. Data are means ± SD (n=8).

 

All Altogen® In Vivo Transfection Kits supplied with ready-to-run transfection protocols that eliminate the need for extensive transfection optimization experiments. Read more about in vivo siRNA transfection, research methods and experimental protocols at Altogen's Transfection Resource.

 

Altogen Labs offers contract research studies, including in vivo testing services, xenograft animal models, teratoma formation, tissue targeted siRNA delivery, and in vivo toxicology CRO services.