A549 Transfection Reagent

Catalog # 1705 - 0.5 mL        $ 170.00 Catalog # 1706 - 1.5 mL        $ 305.00 Transfection reagent for A549 cells Kit includes Transfection Enhancer reagent and recommended transfection protocols Efficient transfection of human lung carcinoma cells Protocols provided for transfection of siRNA, DNA and microRNA High cell viability Work in presense of serum Efficient for plasmid DNA/siRNA cotransfection Reproducible transfection Suitable for Reverse Transfection Developed and manufactured by Altogen Biosystems Transfection Efficiency: Reagent exhibits at least 80% transfection efficiency of siRNA delivery. Transfection efficiency was determined by Fluorescent microscopy, real-time RT-PCR, Western blot. Transfection Protocol: Transfection protocols are provided with the purchase of the reagent.

DATA

Figure 1. SiRNAs targeting Lamin A/C mRNA or non-silencing control siRNA were transfected following the recommended protocol. At 48 hours post-transfection the cells were analyzed by qRT-PCR for gene expression levels. 18S rRNA levels were used to normalize the Lamin A/C data. Values are normalized to untreated sample. Data are means ± SD (n=3).

siRNA Transfection

Gene silencing by RNA Interference (RNAi) is a powerful research tool for studying gene function in mammalian cells. RNAi is a biological phenomenon by which double stranded RNA (dsRNA) specifically reduces gene expression of its corresponding gene. Potent inhibition of specific gene expression is experimentally achieved by the transfection of small interfering RNA (siRNA), which binds RISC complex and cause degradation of target complementary mRNA molecules in the cell. Therefore, successful, potent RNAi experimentation is dependent upon the highly efficient delivery of the siRNA into cells by transfection of stable and functional siRNA molecules. Most DNA transfection reagents are incompatible with siRNA, sensitive to serum, or are capable of inducing cytotoxicity, which negatively affects gene expression studies. Transfection Reagents have been developed and optimized specifically for use with siRNA transfection and allow transfection in the presence of serum without lowering transfection efficiency or reducing cell viability. Ready-to-run transfection protocols eliminate the need for extensive siRNA optimization experiments. Altogen Biosystems kits are also compatible for transfection of microRNA (miRNA) molecules.

Product Review

Article by Dr.Lakshmanan "A549 Transfection Reagent From Altogen Biosystems": Link

A549 (human lung carcinoma cells)

A549 was derived in 1972 from the lung carcinomatous tissue of a 58-year-old Caucasian male. A549 cells are adenocarcinomic human alveolar basal epithelial cells and are squamous in nature and responsible for the diffusion of substances such as water and electrolytes, across the alveoli of lungs. This is a hypotriploid human cell line with the modal chromosome number of 66, occurring in 24 percent of cells. There were also frequent instances of cells with 64, 65, and 67 chromosome counts. A549 cells have been shown to synthesize lecithin with a high percentage of desaturated fatty acids via the cytidine diphosphocholine pathway. These cells are used in vitro model for a type II pulmonary epithelial cell model for drug metabolism. Immunoperoxidase staining shows that A549 cells are positive for keratin. This cell line is typically used to investigate a numerous respiratory ailments, such as viral infections that can cause asthma, tissue damage linked to asbestos exposure, and smoking-related emphysema.

Transfection Resource

siRNA Transfection || Transfection Methods || Stable Transfection || RNAi || siRNA Library Screening || RNAi Therapeutics || Cell Transfection || In Vivo Transfection Reagents || Cell Line Specific Transfection Reagents

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Altogen Research Services

Altogen Custom Services provide specialized biotechnology and pharmaceutical services, including - generation of stable cell lines, RNA Interference (RNAi) services, assay development, screening and transfection services. Generation of stably-expressing cancer cell lines and primary cells can be very expensive and time-consuming. Altogen Services offers generation of stable cell lines by transforming the cell line of choice to stably express gene of interest or shRNA-encoding construct. Cloning and shRNA expression services are available.