Human Aortic Smooth Muscle Cells Transfection Reagent

Catalog # 1707 - 0.5 mL        $ 147.00 Catalog # 1708 - 1.5 mL        $ 364.00 Transfection reagent for ASMC cells Kit includes Transfection Enhancer reagent and recommended transfection protocols Protocols provided for transfection of siRNA and microRNA Optimized, highly effective transfection High cell viability Compatible with transfection in any plate format Optimized for Human Aortic Smooth Muscle Cells Suitable for reverse transfection Developed and manufactured by Altogen Biosystems Transfection Efficiency: Reagent exhibits at least 70% transfection efficiency of siRNA delivery. Transfection efficiency was determined by real-time RT-PCR. Transfection Protocol: Transfection protocols are provided with the purchase of the reagent.

DATA

Figure 1. GAPD mRNA levels were quantified using real-time RT-PCR in the cells transfected with siRNAs targeting GAPD or non-silencing siRNA. Forty-eight hours post-transfection, the cells were harvested and analyzed by real-time RT-PCR for GAPDH mRNA expression levels. Data were normalized against the 18S rRNA signal. Control samples were either mock-transfected or untreated. Values are normalized to untreated sample. Data are means ± SD (n=3).

Transfection Resource

Visit Altogen Biosystems Transfection Resource: RNAi applications (siRNA transfection, microRNA transfection, plasmid DNA transfection), stable and transient DNA transfection, delivery methods, electroporation, microinjection, dendrimer-based, non-viral and virus-mediated gene delivery, liposome and non-liposomal reagents. Transfection of cancer cell lines vs primary cultures. RNA Interference as a research tool for gene expression studies, gene silencing in neuronal cells, RNAi therapeutics and In Vivo Transfection Reagents from Altogen Biosystems.

Human Aortic Smooth Muscle Cells (HAOSMC)

Human Aortic Smooth Muscle Cells are derived from tunica intima and tunica media of plaque-free aorta tissue. The tunica intima is the inner layer of the artery comprised of a lining, a network of connective tissue, and a layer of elastic fibers; the tunica media, or middle coat, is comprised mostly of smooth muscle cells and elastic fibers arranged in spiral layers. Human srterial smooth muscle cells are able to synthesize collagen, elastin, myosin, and glycosaminoglycan. ASMC cells respond to various factors by proliferating or differentiating. These cells are used to study of human vascular disorders such as atherosclerosis, angiogenesis, stroke, vascular biology and also diabetes. Human aortic smooth muscle cells are insulin resistant at the receptor level but sensitive to IGF1 and IGF2. Curcumin prevents the migration of human aortic smooth muscle cells by inhibiting of MMP-9 expression.

Transfection Resource

siRNA Transfection || Transfection Methods || Stable Transfection || RNAi || siRNA Library Screening || RNAi Therapeutics || Cell Transfection || In Vivo Transfection Reagents || Cell Line Specific Transfection Reagents

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Altogen Research Services

Altogen Custom Services provide specialized biotechnology and pharmaceutical services, including - generation of stable cell lines, RNA Interference (RNAi) services, assay development, screening and transfection services. Generation of stably-expressing cancer cell lines and primary cells can be very expensive and time-consuming. Altogen Services offers generation of stable cell lines by transforming the cell line of choice to stably express gene of interest or shRNA-encoding construct. Cloning and shRNA expression services are available.