AsPC-1 Transfection Reagent

Catalog # 1709 - 0.5 mL        $ 132.00 Catalog # 1710 - 1.5 mL        $ 291.00 Transfection reagent for AsPC-1 cells Kit includes Transfection Enhancer reagent and recommended transfection protocols Protocols provided for transfection of mRNA, siRNA and microRNA High transfection efficiency in Human Pancreatic Cells Work in presence of serum High cell viability Compatible with transfection in any plate format Reproducible transfection Developed and manufactured by Altogen Biosystems Transfection Efficiency: Reagent exhibits at least 70% transfection efficiency of siRNA delivery. Transfection efficiency was determined by real-time RT-PCR, Western blot. Transfection Protocol: Transfection protocols are provided with the purchase of the reagent. Shipping information: US orders are shipped within 1-2 business days via UPS Express. International orders are shipped within 3-5 business days (Europe), and 4-9 business days (Worldwide). For maximum stability, store all reagents at +4C upon receipt. US customers: Shipping charge of $20.00 will be added during checkout. Non-US customers: Shipping charge of $20.00 will be added during checkout.

DATA

Figure 1. Cyclophilin B silencing efficiency was determined by qRT-PCR in the cells transfected by Cyclophilin B siRNA or non-silencing siRNA control following the recommended transfection protocol. Cyclophilin mRNA expression levels were measured 48 hours post-transfection. 18S rRNA levels were used to normalize the Cyclophilin B data. Values are normalized to untreated sample. Data are presented as means ± SD (n=3).

Human Pancreatic Cells

AsPC-1 was reestablished via nude mouse xenografts initiated with cells from the ascites of a 62 year-old Caucasian woman with metastatic pancreatic adenocarcinoma. The reestablished epithelial cells retained their original morphology. This tumor represents a new model for human pancreatic cancer. AsPC-1 cells are used in studies seeking possible treatments for pancreatic cancer, which has a five year survival rate of less than one percent. For examples, research has show that the plant alkaloid sanguinarine induced apoptosis of AsPC-1 cells. In another study, immunoprecipitation and ELISA using human pancreatic cancer cells showed the presence of high levels of DcR3 protein in AsPC-1 cells. Reducing endogenous DcR3 levels increases FasL-induced apoptosis of human pancreatic cancer cells. Another intriguing study showed that deuterium oxide (D2O), also called heavy water, induced apoptosis of AsPC-1, and is considered a possible future treatment against AsPC-1.

Altogen Biosystems

Altogen Biosystems is a life sciences company dedicated to the development, marketing and manufacture of cell type specific transfection reagents. Efficient delivery of DNA and RNA molecules (siRNA, plasmid DNA, mRNA, microRNA) enabled by advanced formulation of reagents and peculiar design of transfection protocols. Altogen Biosystems offers a complete transfection system for a broad range of cell lines and primary cells. Reagents are functionally tested to be reproducible, serum compatible, induce low toxicity, and can be used for co-transfection experiments, and high throughput applications.

Transfection Resource

siRNA Transfection || Transfection Methods || Stable Transfection || RNAi || siRNA Library Screening || RNAi Therapeutics || Cell Transfection || In Vivo Transfection Reagents || Cell Line Specific Transfection Reagents

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Altogen Research Services

Altogen Custom Services provide specialized biotechnology and pharmaceutical services, including - generation of stable cell lines, RNA Interference (RNAi) services, assay development, screening and transfection services. Generation of stably-expressing cancer cell lines and primary cells can be very expensive and time-consuming. Altogen Services offers generation of stable cell lines by transforming the cell line of choice to stably express gene of interest or shRNA-encoding construct. Cloning and shRNA expression services are available.