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Astrocytes Transfection Reagent |
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Catalog # 1711 - 0.5 mL $ 163.00
Catalog # 1712 - 1.5 mL $ 339.00
Transfection reagent for Astrocyte cells
- Protocols provided for transfection of DNA, mRNA, siRNA and microRNA
- Kit includes Transfection Enhancer reagent and recommended transfection protocols
- Effective for plasmid DNA/siRNA co-transfection
- Work in presence of serum
- Ideal for primary cultures
- Reproducible transfection
- Cost-effective transfection
- Low cytotoxicity
- Developed and manufactured by Altogen Biosystems
Transfection Efficiency:
Reagent exhibits at least 70% transfection
efficiency of siRNA delivery. Transfection efficiency
was determined by qRT-PCR.
Transfection Protocol:
Transfection protocols are provided with
the purchase of the reagent.
Shipping information:
US orders are shipped within 1-2 business
days via UPS Express.
International orders are shipped within 3-5 business days (Europe), and 4-9
business days (Worldwide).For maximum stability, store all
reagents at +4C upon receipt.
US customers: Shipping charge of $20.00 will be added
during checkout.
Non-US customers: Shipping
charge of $20.00 will be added during checkout.
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DATA |
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Figure 1. SiRNAs targeting Lamin A/C mRNA or non-silencing control siRNA were transfected following the recommended protocol. At 48 hours post-transfection the cells were analyzed by qRT-PCR for gene expression levels. 18S rRNA levels were used to normalize the Lamin A/C data. Values are normalized to untreated sample. Data are means ± SD (n=3).
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Cancer Cell Lines and Primary Cells |
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Primary cell cultures are used in biological and gene therapy studies and serve as important model systems that may more accurately represent the biology of normal cells. Many cultured cell lines, as well as the majority of primary cell cultures are able to be transfected with exogenous nucleic acids when appropriate transfection approaches are employed. Since the majority of transfection methods causes significant toxicity in primary cell cultures, optimizing this procedure, specifically the protocol and reagents to be utilized, is essential for developing an effective transfection strategies for a given cell type. ALTOGENŽ Kits for primary cells and sensitive cell lines have been designed to have significantly lower cytotoxicity than classical alternatives.
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