Astrocytes Transfection Reagent

Catalog # 1711 - 0.5 mL        $ 163.00 Catalog # 1712 - 1.5 mL        $ 339.00 Transfection reagent for Astrocyte cells Protocols provided for transfection of DNA, mRNA, siRNA and microRNA Kit includes Transfection Enhancer reagent and recommended transfection protocols Effective for plasmid DNA/siRNA co-transfection Work in presence of serum Ideal for primary cultures Reproducible transfection Cost-effective transfection Low cytotoxicity Developed and manufactured by Altogen Biosystems Transfection Efficiency: Reagent exhibits at least 70% transfection efficiency of siRNA delivery. Transfection efficiency was determined by qRT-PCR. Transfection Protocol: Transfection protocols are provided with the purchase of the reagent.

DATA

Figure 1. SiRNAs targeting Lamin A/C mRNA or non-silencing control siRNA were transfected following the recommended protocol. At 48 hours post-transfection the cells were analyzed by qRT-PCR for gene expression levels. 18S rRNA levels were used to normalize the Lamin A/C data. Values are normalized to untreated sample. Data are means ± SD (n=3).

Cancer Cell Lines and Primary Cells

Primary cell cultures are used in biological and gene therapy studies and serve as important model systems that may more accurately represent the biology of normal cells. Many cultured cell lines, as well as the majority of primary cell cultures are able to be transfected with exogenous nucleic acids when appropriate transfection approaches are employed. Since the majority of transfection methods causes significant toxicity in primary cell cultures, optimizing this procedure, specifically the protocol and reagents to be utilized, is essential for developing an effective transfection strategies for a given cell type. ALTOGENŽ Kits for primary cells and sensitive cell lines have been designed to have significantly lower cytotoxicity than classical alternatives.

Transfection Resource

siRNA Transfection || Transfection Methods || Stable Transfection || RNAi || siRNA Library Screening || RNAi Therapeutics || Cell Transfection || In Vivo Transfection Reagents || Cell Line Specific Transfection Reagents

Featured Products

In Vivo Transfection Reagents || Fibroblast Cells Transfection Reagent || Astrocyte Cells Transfection Reagent || HEK-293 Transfection Reagent || MEF Transfection Reagent || HepG2 Transfection Reagent || CHO Reagent || MDA-MB Transfection Reagent || Cos7 Transfection Reagent || A549 Transfection Reagent || NIH3T3 Reagent || HUVEC Transfection Reagent || LNCaP Transfection Reagent || MCF-7 Reagent || PC-12 Reagent

Astrocytes

Astrocytes are star-shaped glial cells in the brain and spinal cord. Astrocytes are supportive cells and their functions include biochemical support of endothelial cells which form the blood-brain barrier, providing nutrients to the nerve tissue, extracelular K+ level regulation, neurotransmitters removal, and helping repair the brain and spinal cord following traumatic injuries. They are also dependent for myelination and synapses formation. Many primary astrocytes express the intermediate filament glial fibrillary acidic protein, or GFAP. Research indicates that astrocytes communicate with neurons through the release of gliotransmitters via a calcium ion process. There are three types of astrocytes in the central nervous system: fibrous, protoplasmic and radial. It is believed astrocytes cell bodies are separate but and do not overlap.