Calu-6 (renin-expressing cells) Transfection Reagent

Catalog # 1727 - 0.5 mL        $ 79.00 Catalog # 1728 - 1.5 mL        $ 171.00 Transfection reagent for Calu-6 cells Protocols provided for transfection of DNA, microRNA and siRNA High transfection efficiency in human renin-expressing cells (Calu-6) Low toxicity Work in presence of serum Compatible with transfection in any plate format Reproducible transfection Kit includes Transfection Enhancer reagent and recommended transfection protocols Optimized transfection protocols are adapted for use with both standard & reverse transfection methods Developed and manufactured by Altogen Biosystems Transfection Efficiency: Reagent exhibits at least 90% transfection efficiency of siRNA delivery. Transfection efficiency was determined by real-time RT-PCR. Transfection Protocol: Transfection protocols are provided with the purchase of the reagent.

DATA

Figure 1. GAPD mRNA levels were quantified using real-time RT-PCR in the cells transfected with siRNAs targeting GAPD or non-silencing siRNA. Forty-eight hours post-transfection, the cells were harvested and analyzed by real-time RT-PCR for GAPDH mRNA expression levels. Data were normalized against the 18S rRNA signal. Control samples were either mock-transfected or untreated. Values are normalized to untreated sample. Data are means ± SD (n=3).

Human Renin-expressing Cells

The Calu-6 cell line was derived from tissue of a 61 year-old Caucasian female. The epithelial tissue is from an anaplastic carcinoma from lung tissue. The modal chromosome number is 59. Calu-6 cells have been used to better understand the relationship between antimycin A (AMA) and lung cancer cells. It has been suggested in studies that changes of reactive oxygen species (ROS) and glutathione (GSH) affect apoptosis in AMA-treated Calu-6 cells. In addition, using AMA as a mitochondrial electron transport inhibitor decreased the growth of Calu-6 cells by inducing a G1 arrest of the cell cycle and apoptosis. Tiron, a ROS scavenger, protects human lung cancer Calu-6 cells against antimycin A-induced cell death. This cell line has also been used in research seeking to find the mechanism underlying pressure transduction of the renin-angiotensin system by studying the direct application of mechanical stretch to human renin-expressing cells on the release of renin.

Altogen Biosystems

Altogen Biosystems is a life sciences company dedicated to the development, marketing and manufacture of cell type specific transfection reagents. Efficient delivery of DNA and RNA molecules (siRNA, plasmid DNA, mRNA, microRNA) enabled by advanced formulation of reagents and peculiar design of transfection protocols. Altogen Biosystems offers a complete transfection system for a broad range of cell lines and primary cells. Reagents are functionally tested to be reproducible, serum compatible, induce low toxicity, and can be used for co-transfection experiments, and high throughput applications.

Transfection Resource

siRNA Transfection || Transfection Methods || Stable Transfection || RNAi || siRNA Library Screening || RNAi Therapeutics || Cell Transfection || In Vivo Transfection Reagents || Cell Line Specific Transfection Reagents

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Altogen Research Services

Altogen Custom Services provide specialized biotechnology and pharmaceutical services, including - generation of stable cell lines, RNA Interference (RNAi) services, assay development, screening and transfection services. Generation of stably-expressing cancer cell lines and primary cells can be very expensive and time-consuming. Altogen Services offers generation of stable cell lines by transforming the cell line of choice to stably express gene of interest or shRNA-encoding construct. Cloning and shRNA expression services are available.