CFPEo (HTEpC) Transfection Reagent

Catalog # 1729 - 0.5 mL        $ 98.00 Catalog # 1730 - 1.5 mL        $ 215.00 Transfection reagent for CFPEo (HTEpC) cells Protocols provided for transfection of DNA and mRNA Optimezed for Human Tracheal Epithelial Cells Kit includes Transfection Enhancer reagent and recommended transfection protocols Compatible with transfection in any plate format Reproducible transfection results Optimized transfection protocols are adapted for use with both standard & reverse transfection methods Developed and manufactured by Altogen Biosystems Transfection Efficiency: Reagent exhibits at least 35% transfection efficiency. Transfection efficiency was determined by Fluorescent microscopy. Transfection Protocol: Transfection protocols are provided with the purchase of the reagent.

CFPEO (HTEpC) cell line

HTEpC are derived from the surface epithelium of normal human trachea. Epithelial cells in the airways are responsible for lubricating the lungs, maintaining humidity, and cleaning the respiratory tract. HTEpC are an important target for drugs, toxins, and carcinogens. These cells are afflicted in cases of asthma, viral infection, fibrotic lung disease, and cystic fibrosis. Consequently, HTEpC are useful for investigating the function and pathology of the respiratory system. They are also used in drug uptake experiments, toxicological and wound healing research, oncology, and cell differentiation. Investigations include studying the regulation of intracellular pH, the stimulant effect of IL-1b on airway epithelial cell growth, and how human rhinovirus infection affects upregulation, or hastening, of ICAM-1 expression on epithelial cells. HTEpC stain positive for cytokeratin. It has been shown that the HTEpC system can be used in a quality control procedure for testing the cellular compatibility of materials for endotracheal use.

Altogen Biosystems

Altogen Biosystems is a life sciences company dedicated to the development, marketing and manufacture of cell type specific transfection reagents. Efficient delivery of DNA and RNA molecules (siRNA, plasmid DNA, mRNA, microRNA) enabled by advanced formulation of reagents and peculiar design of transfection protocols. Altogen Biosystems offers a complete transfection system for a broad range of cell lines and primary cells. Reagents are functionally tested to be reproducible, serum compatible, induce low toxicity, and can be used for co-transfection experiments, and high throughput applications.

Transfection Resource

siRNA Transfection || Transfection Methods || Stable Transfection || RNAi || siRNA Library Screening || RNAi Therapeutics || Cell Transfection || In Vivo Transfection Reagents || Cell Line Specific Transfection Reagents

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Altogen Research Services

Altogen Custom Services provide specialized biotechnology and pharmaceutical services, including - generation of stable cell lines, RNA Interference (RNAi) services, assay development, screening and transfection services. Generation of stably-expressing cancer cell lines and primary cells can be very expensive and time-consuming. Altogen Services offers generation of stable cell lines by transforming the cell line of choice to stably express gene of interest or shRNA-encoding construct. Cloning and shRNA expression services are available.