CFPEo (HTRpC) Transfection Reagent (Trachea Epithelium)
Transfection Reagent for CFPEo (HTRpC) Cells (Trachea Epithelium Cells)
Proprietary cationic lipids formulation
High transfection efficiency of small RNA (siRNA, shRNA, miRNA), mRNA, pDNA
Effective and robust intracellular delivery
Kit includes Transfection Enhancer reagent
Produces consistent results, lot-to-lot, plate-to-plate, and well-to-well
Work in the presence of serum
A proven reagent for establishing stable cell lines
Optimized transfection protocols are adapted for use with both standard & reverse transfection methods
Developed and manufactured by Altogen Biosystems
Reagent exhibits at least 73% transfection efficiency of siRNA delivery. Transfection efficiency was determined by qRT-PCR.
Transfection Protocol and MSDS:
Download Altogen Biosystems CFPEo Transfection Protocol: [PDF]
Download MSDS: [PDF]
CFPEo (HTRpC) Cell Line:
The CFPEo cell line was established using human trachea epithelium cells (also known as HTEpC Cell Line). These types of cells in the airways are responsible for lubricating the lungs, maintaining humidity, and cleaning the respiratory tract. CFPEo cells are very useful cell culture model in biomedical research for treatments of asthma, viral infection, fibrotic lung disease, lung cancer, and cystic fibrosis.
HTEpC are derived from the surface epithelium of normal human trachea. Epithelial cells in the airways are responsible for lubricating the lungs, maintaining humidity, and cleaning the respiratory tract. HTEpC are an important target for drugs, toxins, and carcinogens. These cells are afflicted in cases of asthma, viral infection, fibrotic lung disease, and cystic fibrosis. Consequently, HTEpC are useful for investigating the function and pathology of the respiratory system. They are also used in drug uptake experiments, toxicological and wound healing research, oncology, and cell differentiation. Investigations include studying the regulation of intracellular pH, the stimulant effect of IL-1b on airway epithelial cell growth, and how human rhinovirus infection affects upregulation, or hastening, of ICAM-1 expression on epithelial cells.
Figure 1. Cyclophilin B silencing efficiency was determined by qRT-PCR in the CFPEO cells transfected by Cyclophilin B siRNA or non-silencing siRNA control following the recommended transfection protocol. Cyclophilin mRNA expression levels were measured 48 hours post-transfection. 18S rRNA levels were used to normalize the Cyclophilin B data. Values are normalized to untreated sample. Data are presented as means ± SD (n=6).
Altogen Biosystems is a life sciences company that offers cell type-specific and pre-optimized transfection products, elecroporation kits, and in vivo delivery reagents (peg-liposome, nanoparticle, lipid, and polymer-based; and tissue targeted: liver, pancreas, kidney). Advanced formulation of reagents and optimized transfection protocols provide efficient intracellular delivery of protein, DNA, mRNA, shRNA and siRNA molecules. Read more about transfection technology at Altogen's Transfection Resource.
Altogen Research Services:
Altogen Labs is a GLP compliant laboratory that provides preclinical contract research services (CRO). Pharma and biotechnology services include: transient and stable transfection (development of stable cell lines), liposome encapsulation (proteins, DNA, mRNA, siRNA), A-to-Z RNAi services, in vivo and in vitro toxicology, xenograft animal models, assay development, cell banking and cryopreservation services.