CHO Transfection Reagent

Catalog # 2055 - 0.5 mL        $ 74.00 Catalog # 2066 - 1.5 mL        $ 187.00 Transfection reagent for CHO cells Kit includes Transfection Enhancer reagent and recommended transfection protocols Protocols provided for transfection of DNA, mRNA, siRNA and microRNA High transfection efficiency Work in presence of serum Optimized for chinese hamster ovary epithelial cells Reproducible transfection Efficient DNA/siRNA cotransfection Developed and manufactured by Altogen Biosystems Transfection Efficiency: Reagent exhibits at least 90% transfection efficiency of siRNA delivery. Transfection efficiency was determined by fluorescent microscopy, real-time RT-PCR. Transfection Protocol: Transfection protocols are provided with the purchase of the reagent.

DATA

Figure 1. GAPD mRNA levels were quantified using real-time RT-PCR in the cells transfected with siRNAs targeting GAPD or non-silencing siRNA. Forty-eight hours post-transfection, the cells were harvested and analyzed by real-time RT-PCR for GAPDH mRNA expression levels. Data were normalized against the 18S rRNA signal. Control samples were either mock-transfected or untreated. Values are normalized to untreated sample. Data are means ± SD (n=3).

Transfection Resource

Visit Altogen Biosystems Transfection Resource: RNAi applications (siRNA transfection, microRNA transfection, plasmid DNA transfection), stable and transient DNA transfection, delivery methods, electroporation, microinjection, dendrimer-based, non-viral and virus-mediated gene delivery, liposome and non-liposomal reagents. Transfection of cancer cell lines vs primary cultures. RNA Interference as a research tool for gene expression studies, gene silencing in neuronal cells, RNAi therapeutics and In Vivo Transfection Reagents from Altogen Biosystems.

Altogen Biosystems

Altogen Biosystems is a life sciences company dedicated to the development, marketing and manufacture of cell type specific transfection reagents. Efficient delivery of DNA and RNA molecules (siRNA, plasmid DNA, mRNA, microRNA) enabled by advanced formulation of reagents and peculiar design of transfection protocols. Altogen Biosystems offers a complete transfection system for a broad range of cell lines and primary cells. Reagents are functionally tested to be reproducible, serum compatible, induce low toxicity, and can be used for co-transfection experiments, and high throughput applications.

Transfection Resource

siRNA Transfection || Transfection Methods || Stable Transfection || RNAi || siRNA Library Screening || RNAi Therapeutics || Cell Transfection || In Vivo Transfection Reagents || Cell Line Specific Transfection Reagents

CHO cell line

Chinese hamster ovary (CHO) cells are a cell line derived from the ovary of the Chinese hamster. The CHO line was established in 1957. The Chinese hamster has a low chromosome number (2n=22) making it an excellent model for radiation cytogenetics and tissue culture. CHO are the most widely used mammalian cells for transfection, expression, and large-scale recombinant protein production. CHO require proline in their culture medium. The CHO cell line has a high cloning efficiency percentage and exceptional colony-forming properties. CHO forms tightly packed cells in readily distinguished colonies that can be counted automatically rather than manually. The cell line is highly sensitive to toxic material. The CHO clonal cytotoxicity test system has been validated through analysis of organic compounds, environmental samples, and metal salts. CHO cells do not express the epidermal growth factor receptor.

Altogen Research Services

Altogen Custom Services provide specialized biotechnology and pharmaceutical services, including - generation of stable cell lines, RNA Interference (RNAi) services, assay development, screening and transfection services. Generation of stably-expressing cancer cell lines and primary cells can be very expensive and time-consuming. Altogen Services offers generation of stable cell lines by transforming the cell line of choice to stably express gene of interest or shRNA-encoding construct. Cloning and shRNA expression services are available.