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Chromaffin cells Transfection Reagent |
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Catalog # 1733 - 0.5 mL $ 104.00
Catalog # 1734 - 1.5 mL $ 285.00
Transfection reagent for Chromaffin cells
- Kit includes Transfection Enhancer reagent and recommended transfection protocols
- Protocols provided for transfection of siRNA, DNA, mRNA and microRNA
- High cell viability
- Suitable for siRNA/DNA cotransfection
- Compatible with transfection in any plate format
- Reproducible results
- Minimal off-target effect
- Developed and manufactured by Altogen Biosystems
Transfection Efficiency:
Reagent exhibits at least 75% transfection
efficiency of siRNA delivery. Transfection efficiency
was determined by real-time RT-PCR.
Transfection Protocol:
Transfection protocols are provided with
the purchase of the reagent.
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DATA |
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Figure 1. Cyclophilin B silencing efficiency was determined by qRT-PCR in the cells transfected by Cyclophilin B siRNA or non-silencing siRNA control following the recommended transfection protocol. Cyclophilin mRNA expression levels were measured 48 hours post-transfection. 18S rRNA levels were used to normalize the Cyclophilin B data. Values are normalized to untreated sample. Data are presented as means ± SD (n=3).
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Cancer Cell Lines and Primary Cells |
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Primary cell cultures are used in biological and gene therapy studies and serve as important model systems that may more accurately represent the biology of normal cells. Many cultured cell lines, as well as the majority of primary cell cultures are able to be transfected with exogenous nucleic acids when appropriate transfection approaches are employed. Since the majority of transfection methods causes significant toxicity in primary cell cultures, optimizing this procedure, specifically the protocol and reagents to be utilized, is essential for developing an effective transfection strategies for a given cell type. ALTOGENŽ Kits for primary cells and sensitive cell lines have been designed to have significantly lower cytotoxicity than classical alternatives.
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