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Cos-7 Transfection Reagent |
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Catalog # 1739 - 0.5 mL $ 97.00
Catalog # 1740 - 1.5 mL $ 229.00
Transfection reagent for Cos-7 cells
- Kit includes Transfection Enhancer reagent and recommended transfection protocols
- Protocols provided for transfection of DNA, mRNA, siRNA and microRNA
- Efficient for DNA/siRNA cotransfection
- Low toxicity
- Optimized for Monkey Kidney Cells
- High knockdown using siRNA with reduced off-target effects
- Optimized transfection protocols are adapted for use with both standard & reverse transfection methods
- Developed and manufactured by Altogen Biosystems
Transfection Efficiency:
Reagent exhibits at least 80% transfection
efficiency of siRNA delivery. Transfection efficiency
was determined by real-time RT-PCR, Western blot.
Transfection Protocol:
Transfection protocols are provided with
the purchase of the reagent.
Shipping information:
US orders are shipped within 1-2 business
days via UPS Express.
International orders are shipped within 3-5 business days (Europe), and 4-9
business days (Worldwide).For maximum stability, store all reagents at +4C upon receipt.
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DATA |
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Figure 1. SiRNAs targeting Lamin A/C mRNA or non-silencing control siRNA were transfected following the recommended protocol. At 48 hours post-transfection the cells were analyzed by qRT-PCR for gene expression levels. 18S rRNA levels were used to normalize the Lamin A/C data. Values are normalized to untreated sample. Data are means ± SD (n=3).
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COS-7 (Monkey Kidney Cells) |
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COS-7 was established from a CV-1 line derived from kidney cells of the African green monkey (Cercopithecus aethiops) through transformation with a mutant version of the SV-40 genome that can produce large T antigen. They are fibroblast-like cells growing as monolayers. The COS-7 cell line is frequently used by researchers to study the SV-40 monkey virus. It is also used to transfect cells to produce recombinant proteins for biochemistry, molecular and cellular biology experiments. COS-7 has been shown to retain complete permissiveness for lytic growth of SV40, support the replication of tsA209 virus at 40°C, and support the replication of pure populations of SV40 mutants. COS-7 is designated as a risk category 1 according to the German Central Commission for Biological Safety (ZKBS).
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