DI-TNC1 Transfection Reagent

Catalog # 1747 - 0.5 mL        $ 141.00 Catalog # 1748 - 1.5 mL        $ 319.00 Transfection reagent for DI-TNC1 cells Protocols provided for transfection of siRNA, mRNA and microRNA Kit includes Transfection Enhancer reagent and recommended transfection protocols Work in presence of serum Compatible with transfection in any plate format Reproducible transfection results Optimized transfection of rat brain astrocyte cells Developed and manufactured by Altogen Biosystems Transfection Efficiency: Reagent exhibits at least 70% transfection efficiency of siRNA delivery. Transfection efficiency was determined by Fluorescent microscopy, real-time RT-PCR, Western blot. Transfection Protocol: Transfection protocols are provided with the purchase of the reagent.

Transfection Resource

Visit Altogen Biosystems Transfection Resource: RNAi applications (siRNA transfection, microRNA transfection, plasmid DNA transfection), stable and transient DNA transfection, delivery methods, electroporation, microinjection, dendrimer-based, non-viral and virus-mediated gene delivery, liposome and non-liposomal reagents. Transfection of cancer cell lines vs primary cultures. RNA Interference as a research tool for gene expression studies, gene silencing in neuronal cells, RNAi therapeutics and In Vivo Transfection Reagents from Altogen Biosystems.

Altogen Biosystems

Altogen Biosystems is a life sciences company dedicated to the development, marketing and manufacture of cell type specific transfection reagents. Efficient delivery of DNA and RNA molecules (siRNA, plasmid DNA, mRNA, microRNA) enabled by advanced formulation of reagents and peculiar design of transfection protocols. Altogen Biosystems offers a complete transfection system for a broad range of cell lines and primary cells. Reagents are functionally tested to be reproducible, serum compatible, induce low toxicity, and can be used for co-transfection experiments, and high throughput applications.

Transfection Resource

siRNA Transfection || Transfection Methods || Stable Transfection || RNAi || siRNA Library Screening || RNAi Therapeutics || Cell Transfection || In Vivo Transfection Reagents || Cell Line Specific Transfection Reagents

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Rat brain astrocyte cells

DI-TNC1 was derived from cultures of interbrain tissue in one day old rats. Cultured astrocytes have been shown to promote neurite outgrowth by producing adhesion molecules found either on the cell surface or in the extracellular matrix. The cells retain characteristics of type 1 astrocytes including glial fibrillary acidic protein (GFAP) immunoreactivity. DI-TNC1 produces alpha 2 macroglobulin similar to amounts found in primary astrocytes but produce transferrin in much lesser amounts. Examination by immunostaining indicated SV40 T antigen was found in the nuclei of over 95 percent of the cells. Research has shown DI-TNC1 cells show many similarities to neonatal astrocytes and the cell line may be useful in future studies on interactions between glia and neurons. DI-TNC1 cells may also be used to help decipher some of the astrocytic functions related to energy metabolism.