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DU145 Transfection Reagent |
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Catalog # 1749 - 0.5 mL $ 117.00
Catalog # 1750 - 1.5 mL $ 267.00
Transfection reagent for DU145 cells
- For human prostate cancer cells (DU-145)
- Protocols provided for transfection of DNA, mRNA and microRNA
- Reproducible and reliable results
- Work in presence of serum
- Compatible with transfection in any plate format
- Reproducible transfection
- Optimized transfection using DU145 cell-specific protocol
- Developed and manufactured by Altogen Biosystems
Transfection Efficiency:
Reagent exhibits at least 60% transfection
efficiency. Transfection efficiency
was determined by fluorescent microscopy.
Transfection Protocol:
Transfection protocols are provided with
the purchase of the reagent.
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DU145 Cell Line |
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DU 145 was derived in 1975 from a brain lesion in a 69 year-old man with metastatic carcinoma of the prostate and a three year history of lymphocytic leukemia. They are epithelial-like adherent cells growing as monolayers. The DU-145 line is not measurably hormone sensitive. It is only weakly positive for acid phosphatase and isolated cells form colonies in soft agar. Analyses of the cell line has revealed microvilli, tonofilaments and desmosomes. DU-145 do not express prostate antigen but do express functional receptor activator of NFkappaB and androgen receptor. The DU-145 cell line is mostly used in laboratory situations to study the way prostate cancer cells grow. It has been shown glucosamine suppresses proliferation of DU-145.
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Altogen Research Services |
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Altogen Custom Services provide specialized biotechnology and pharmaceutical services, including - generation of stable cell lines, RNA Interference (RNAi) services, assay development, screening and transfection services. Generation of stably-expressing cancer cell lines and primary cells can be very expensive and time-consuming. Altogen Services offers generation of stable cell lines by transforming the cell line of choice to stably express gene of interest or shRNA-encoding construct. Cloning and shRNA expression services are available.
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