PEG-Liposome siRNA In Vivo Transfection Kit From Altogen Biosystems

LAS VEGAS, Oct. 21 -- Altogen Biosystems announced the release of its second generation of in vivo RNAi transfection reagent in the company's popular in vivo delivery product line: PEG-Liposome siRNA In Vivo Transfection Kit. This reagent is also compatible for in vivo transfection of other negatively charged molecules – RNA, DNA, and small proteins. The company also offers custom encapsulation of target molecules into PEG-liposome in vivo transfection reagent.

Novel PEGylated liposome-based siRNA delivery kit is optimized for directed RNAi induction by efficient delivery of functional small RNA molecules (siRNA, shRNA, miRNA) into tissues. Liposome component provides in vivo siRNA protection due to efficient siRNA-liposome encapsulation. A remarkable feature of this reagent is reduced innate immune response and low cytotoxicity due to biodegradable PEG modification.

Altogen Biosystems has previously developed nanoparticle-, polymer- and lipid-based in vivo transfection systems (www.altogen.com/mirna.htm) used for laboratory testing pharmaceuticals in cells and animals.

Altogen Biosystems is focusing on commercializing pharma- and bio-technologies, as well as providing custom laboratory services. The company offers pre-clinical contract research services (CRO) including Pharmacology/Toxicology studies, assay development, molecular and cell biology services, development of stable cell lines, gene silencing and complete RNAi services (www.altogenservices.com).

Stable Cell Line Generation and RNAi Services

Stable Cell Line Production in 28 Days

Altogen Biosystems offers contract research services including the generation of stable cell lines, vector construction, and RNAi cell transfection services. The generation of cells permanently expressing a gene of interest or siRNA-expressing construct can be very complex, expensive, and time-consuming. Altogen Custom Services utilizes its unique experience with over 100 cancer cell lines and its expertise in polymer- and nanoparticle-based gene delivery technologies to produce stable clonal cells in just 28 days. Other custom services include assay development, cloning, transient transfection, shRNA-encoding vector construction, siRNA design, and validation in vitro.

 

In Vivo Transfection Reagents

New Generation In Vivo Transfection Reagents

Altogen Biosystems offers various types of in vivo transfection reagents used for testing pharmaceuticals in animals. The major challenge in performing RNAi studies in vivo is the efficient, directed delivery of functional siRNA, shRNA, and microRNA molecules into specific tissues. Lipid-, polymer-, and nanoparticle-based in vivo transfection reagents provide differential transfection efficiency to particular organs and tumor tissues. All in vivo transfection reagents are stable in serum, enhance delivery efficiency, and provide a prolonged, siRNA-induced, gene silencing effect. Reagents are available for systemic administration as well as for direct intratumoral and intraperitoneal injections.

 

Pre-optimized Transfection Reagents

Cell Line - Specific Transfection Reagents

Altogen Biosystems is a leading manufacturer and developer of cell type-specific, pre-optimized transfection kits and products. Reagents are designed for individual cancer cell lines and many primary cell types and are capable of delivering functional plasmid DNA, mRNA, siRNA, and microRNA molecules into cells. Most DNA transfection reagents are incompatible with siRNA, are sensitive to serum, or are capable of inducing cytotoxicity, which negatively affects gene expression studies. ALTOGEN® Transfection Reagents have been developed and optimized specifically for use with siRNA transfection methods and allow transfection in the presence of serum without either lowering transfection efficiency or reducing cell viability. Ready-to-run transfection protocols eliminate the need for extensive transfection optimization experiments. Transfection kits include cell type-specific transfection protocols and a transfection enhancer reagent. Transfection kits are highly efficient, cost-effective, cell type-specific, reverse transfection compatible, and provide high reproducibility.

 

Transfection Resource

siRNA Transfection || Transfection Methods || Stable Transfection || RNAi || siRNA Library Screening || RNAi Therapeutics || Cell Transfection || In Vivo Transfection Reagents || Cell Line Specific Transfection Reagents

Featured Products

In Vivo Transfection Reagents || Fibroblast Cells Transfection Reagent || Astrocyte Transfection Reagent || HEK-293 Transfection Reagent || MEF Transfection Reagent || HUVEC Transfection Reagent || LNCaP Transfection Reagent || MCF-7 Reagent || PC-12 Reagent

Altogen Research Services

Altogen Custom Services provide specialized biotechnology and pharmaceutical services, including - generation of stable cell lines, RNA Interference (RNAi) services, assay development, screening and transfection services. Generation of stably-expressing cancer cell lines and primary cells can be very expensive and time-consuming. Altogen Services offers generation of stable cell lines by transforming the cell line of choice to stably express gene of interest or shRNA-encoding construct. Cloning and shRNA expression services are available.