Fibroblasts Transfection Reagent

Catalog # 1755 - 0.5 mL        $ 127.00 Catalog # 1756 - 1.5 mL        $ 270.00 Transfection reagent for Fibroblast cells Transfection Enhancer reagent included Optimized easy-to-use transfection protocol provided for transfection of DNA, mRNA, siRNA and microRNA Ideal for primary fibroblast cells Work in presence of serum Reproducible transfection results Works well for reverse transfection and high throughput applications Developed and manufactured by Altogen Biosystems Transfection Efficiency: Reagent exhibits at least 85% transfection efficiency of siRNA delivery. Transfection efficiency was determined by real-time RT-PCR. Transfection Protocol: Transfection protocols are provided with the purchase of the reagent.

DATA

Figure 1. SiRNAs targeting Lamin A/C mRNA or non-silencing control siRNA were transfected following the recommended protocol. At 48 hours post-transfection the cells were analyzed by qRT-PCR for gene expression levels. 18S rRNA levels were used to normalize the Lamin A/C data. Values are normalized to untreated sample. Data are means ± SD (n=3).

Fibroblast cell lines and Primary cells

Fibroblasts maintain the structure of connective tissues by secreting precursors of the extracellular matrix, primarily a variety of fibres and the ground substance. · Fibroblasts and related connective tissues sculpt the major portion of an organism. · Fibroblasts are derived from primitive mesenchyme. · Fibroblasts have a branched cytoplasm that surrounds a nucleus with one or two nucleoli. · Fibroblasts can migrate slowly over substratum as individual cells (in contrast to epithelial cells). · Fibroblasts can show dissimilar phenotypes in different anatomical locations. · Active fibroblasts have rough abundant endoplasmic reticulum. · Fibroblasts often align in parallel clusters when crowded. · Unlike the epithelial cells lining the body structures, fibroblasts don’t form flat mono-layers and are not restricted to a basal lamina on one side. They can contribute to basal lamina components in some situations. For more information on Fibroblast cells visit Wikipedia and WikiGenes.

Altogen Biosystems

Altogen Biosystems is a life sciences company dedicated to the development, marketing and manufacture of cell type specific transfection reagents. Efficient delivery of DNA, RNA, siRNA, and microRNA enabled by advanced formulation of reagents and peculiar design of protocols. Altogen Biosystems offers a complete transfection system for a broad range of cell lines. All reagents are functionally tested to be highly reproducible, serum compatible, induce low toxicity, and can be used for co-transfection experiments, and high throughput applications.

Transfection Resource

siRNA Transfection || Transfection Methods || Stable Transfection || RNAi || siRNA Library Screening || RNAi Therapeutics || Cell Transfection || In Vivo Transfection Reagents || Cell Line Specific Transfection Reagents

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Altogen Research Services

Altogen Custom Services provide specialized biotechnology and pharmaceutical services, including - generation of stable cell lines, RNA Interference (RNAi) services, assay development, screening and transfection services. Generation of stably-expressing cancer cell lines and primary cells can be very expensive and time-consuming. Altogen Services offers generation of stable cell lines by transforming the cell line of choice to stably express gene of interest or shRNA-encoding construct. Cloning and shRNA expression services are available.