Fibroblast Transfection Reagent (Fibroblast Cells)
Transfection Reagent for Fibroblast Cells (Fibroblast Cell Lines and Primary Fibroblasts)
- Proprietary cationic lipids formulation
High transfection efficiency of small RNA (siRNA, shRNA, miRNA), mRNA, pDNA
Effective and robust intracellular delivery
Kit includes Transfection Enhancer reagent
Produces consistent results, lot-to-lot, plate-to-plate, and well-to-well
Work in the presence of serum
A proven reagent for establishing stable cell lines
Optimized transfection protocols are adapted for use with both standard & reverse transfection methods
Developed and manufactured by Altogen Biosystems
Reagent exhibits at least 85% transfection efficiency of siRNA delivery. Transfection efficiency was determined by qRT-PCR.
Transfection Protocol and MSDS:
Download Altogen Biosystems Fibroblasts Transfection Protocol: [PDF]
Download MSDS: [PDF]
Fibroblast Cell Lines and Primary Fibroblasts:
Fibroblasts maintain the structure of connective tissues by secreting precursors of the extracellular matrix, primarily a variety of fibres and the ground substance. Fibroblasts and related connective tissues sculpt the major portion of an organism. Fibroblasts are derived from primitive mesenchyme. Fibroblasts have a branched cytoplasm that surrounds a nucleus with one or two nucleoli. Fibroblasts can migrate slowly over substratum as individual cells (in contrast to epithelial cells). Fibroblasts can show dissimilar phenotypes in different anatomical locations. Active fibroblasts have rough abundant endoplasmic reticulum. Fibroblasts often align in parallel clusters when crowded. Unlike the epithelial cells lining the body structures, fibroblasts don’t form flat mono-layers and are not restricted to a basal lamina on one side. They can contribute to basal lamina components in some situations.
Figure 1. SiRNAs targeting Lamin A/C mRNA or non-silencing control siRNA were transfected into fibroblast cells following the recommended protocol. At 48 hours post-transfection the cells were analyzed by qRT-PCR for gene expression levels. 18S rRNA levels were used to normalize the Lamin A/C data. Values are normalized to untreated sample. Data are means ± SD (n=3).
Altogen Biosystems is a life sciences company that offers cell type-specific and pre-optimized transfection products, elecroporation kits, and in vivo delivery reagents (peg-liposome, nanoparticle, lipid, and polymer-based; and tissue targeted: liver, pancreas, kidney). Advanced formulation of reagents and optimized transfection protocols provide efficient intracellular delivery of protein, DNA, mRNA, shRNA and siRNA molecules. Read more about transfection technology at Altogen's Transfection Resource.
Altogen Research Services:
Altogen Labs is a GLP compliant laboratory that provides preclinical contract research services (CRO). Pharma and biotechnology services include: transient and stable transfection (development of stable cell lines), liposome encapsulation (proteins, DNA, mRNA, siRNA), A-to-Z RNAi services, in vivo and in vitro toxicology, xenograft animal models, assay development, cell banking and cryopreservation services.