HCS-2 Transfection Reagent (Chondrosarcoma Cells)

Catalog #1761 HCS-2 Transfection Reagent, Size: 0.5 mL $136

Catalog #1762 HCS-2 Transfection Reagent, Size: 1.5 mL $318

Transfection Reagent for HCS-2 Cells (Chondrosarcoma Cells)

  • Proprietary cationic lipids formulation
  • High transfection efficiency of small RNA (siRNA, shRNA, miRNA), mRNA, pDNA
  • Effective and robust intracellular delivery
  • Kit includes Transfection Enhancer reagent
  • Produces consistent results, lot-to-lot, plate-to-plate, and well-to-well
  • Work in the presence of serum
  • A proven reagent for establishing stable cell lines
  • Optimized transfection protocols are adapted for use with both standard & reverse transfection methods
  • Download in vitro HCS2 transfection protocol: [PDF]
  • Developed and manufactured by Altogen Biosystems

Transfection Efficiency:

Reagent exhibits at least 80% transfection efficiency of siRNA delivery. Transfection efficiency was determined by qRT-PCR.

Transfection Protocol and MSDS:

Download Altogen Biosystems HCS-2 Transfection Protocol: [PDF]

Download MSDS: [PDF]

HCS-2 Cell Line:

The HCS-2 cell line was established from human chondrosarcoma cells. HCS-2 cells are widely used in cartilage biomedical research. Medical research for patients with certain types of sarcoma (cancer) often use this cell line as a biolgical model. Chondrosarcoma comprises ~30% of all skeletal system cancers, and affect primarily the axial skeleton (bones of the skull, vertebra, and ribs) of older individuals.

Data:

Figure 1. Cyclophilin B silencing efficiency was determined by qRT-PCR in the HCS-2 cells transfected by Cyclophilin B siRNA or non-silencing siRNA control following the recommended transfection protocol. Cyclophilin mRNA expression levels were measured 48 hours post-transfection. 18S rRNA levels were used to normalize the Cyclophilin B data. Values are normalized to untreated sample. Data are presented as means ± SD (n=3).

Altogen Biosystems:

Altogen Biosystems is a life sciences company that offers cell type-specific and pre-optimized transfection products, elecroporation kits, and in vivo delivery reagents (peg-liposome, nanoparticle, lipid, and polymer-based; and tissue targeted: liver, pancreas, kidney). Advanced formulation of reagents and optimized transfection protocols provide efficient intracellular delivery of protein, DNA, mRNA, shRNA and siRNA molecules. Read more about transfection technology at Altogen's Transfection Resource.    

Altogen Research Services:

Altogen Labs is a GLP compliant laboratory that provides preclinical contract research services (CRO). Pharma and biotechnology services include: transient and stable transfection (development of stable cell lines), liposome encapsulation (proteins, DNA, mRNA, siRNA), A-to-Z RNAi services, in vivo and in vitro toxicology, xenograft animal models, assay development, cell banking and cryopreservation services.