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HEK-293 Transfection Reagent |
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Catalog # 1763 - 0.5 mL $ 112.00
Catalog # 1764 - 1.5 mL $ 258.00
Transfection reagent for HEK-293 cells
- Kit includes Transfection Enhancer reagent and recommended transfection protocols
- Protocols provided for transfection of DNA, mRNA, siRNA and microRNA
- High cell viability
- Efficient plasmid DNA/siRNA cotransfection
- Compatible with Reverse Transfection
- Reliable results
- Optimized protocol for transfection of epithelial kidney cells
- Developed and manufactured by Altogen Biosystems
Transfection Efficiency:
Reagent exhibits at least 90% transfection
efficiency of siRNA delivery. Transfection efficiency
was determined by real-time RT-PCR, Western blot.
Transfection Protocol:
Transfection protocols are provided with
the purchase of the reagent.
Shipping information:
US orders are shipped within 1-2 business
days via UPS Express.
International orders are shipped within 3-5 business days (Europe), and 4-9
business days (Worldwide). For maximum stability, store all
reagents at +4C upon receipt.
US customers: Shipping charge of $20.00 will be added
during checkout.
Non-US customers: Shipping
charge of $20.00 will be added during checkout.
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DATA |
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Figure 1. SiRNAs targeting Lamin A/C mRNA or non-silencing control siRNA were transfected following the recommended protocol. At 48 hours post-transfection the cells were analyzed by qRT-PCR for gene expression levels. 18S rRNA levels were used to normalize the Lamin A/C data. Values are normalized to untreated sample. Data are means ± SD (n=3).
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siRNA Transfection |
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Gene silencing by RNA Interference (RNAi) is a powerful research tool for studying gene function in mammalian cells. RNAi is a biological phenomenon by which double stranded RNA (dsRNA) specifically reduces gene expression of its corresponding gene. Potent inhibition of specific gene expression is experimentally achieved by the transfection of small interfering RNA (siRNA), which binds RISC complex and cause degradation of target complementary mRNA molecules in the cell. Therefore, successful, potent RNAi experimentation is dependent upon the highly efficient delivery of the siRNA into cells by transfection of stable and functional siRNA molecules.
Most DNA transfection reagents are incompatible with siRNA, sensitive to serum, or are capable of inducing cytotoxicity, which negatively affects gene expression studies. Transfection Reagents have been developed and optimized specifically for use with siRNA transfection and allow transfection in the presence of serum without lowering transfection efficiency or reducing cell viability. Ready-to-run transfection protocols eliminate the need for extensive siRNA optimization experiments. Altogen Biosystems kits are also compatible for transfection of microRNA (miRNA) molecules.
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