HEK-293 Transfection Reagent

Catalog # 1763 - 0.5 mL        $ 112.00 Catalog # 1764 - 1.5 mL        $ 258.00 Transfection reagent for HEK-293 cells For human Epithelial Kidney Cells Kit includes Transfection Enhancer reagent and recommended transfection protocols Protocols provided for transfection of DNA, mRNA, siRNA and microRNA High cell viability Efficient plasmid DNA/siRNA cotransfection Compatible with Reverse Transfection Reliable results Optimized protocol for transfection of epithelial kidney cells Developed and manufactured by Altogen Biosystems Transfection Efficiency: Reagent exhibits at least 90% transfection efficiency of siRNA delivery. Transfection efficiency was determined by real-time RT-PCR, Western blot. Transfection Protocol: Transfection protocols are provided with the purchase of the reagent.

DATA

Figure 1. SiRNAs targeting Lamin A/C mRNA or non-silencing control siRNA were transfected following the recommended protocol. At 48 hours post-transfection the cells were analyzed by qRT-PCR for gene expression levels. 18S rRNA levels were used to normalize the Lamin A/C data. Values are normalized to untreated sample. Data are means ± SD (n=3).

siRNA Transfection

Gene silencing by RNA Interference (RNAi) is a powerful research tool for studying gene function in mammalian cells. RNAi is a biological phenomenon by which double stranded RNA (dsRNA) specifically reduces gene expression of its corresponding gene. Potent inhibition of specific gene expression is experimentally achieved by the transfection of small interfering RNA (siRNA), which binds RISC complex and cause degradation of target complementary mRNA molecules in the cell. Therefore, successful, potent RNAi experimentation is dependent upon the highly efficient delivery of the siRNA into cells by transfection of stable and functional siRNA molecules. Most DNA transfection reagents are incompatible with siRNA, sensitive to serum, or are capable of inducing cytotoxicity, which negatively affects gene expression studies. Transfection Reagents have been developed and optimized specifically for use with siRNA transfection and allow transfection in the presence of serum without lowering transfection efficiency or reducing cell viability. Ready-to-run transfection protocols eliminate the need for extensive siRNA optimization experiments. Altogen Biosystems kits are also compatible for transfection of microRNA (miRNA) molecules.

Transfection Resource

siRNA Transfection || Transfection Methods || Stable Transfection || RNAi || siRNA Library Screening || RNAi Therapeutics || Cell Transfection || In Vivo Transfection Reagents || Cell Line Specific Transfection Reagents

HEK293 Cell Line

HEK-293 (human epithelial kidney cells) were originally derived in 1977 from human embryonic kidney cells transformed with sheared adenovirus 5 DNA grown in tissue culture. The transformation incorporated approximately 4.5 kilobases from the viral genome into human chromosome 19 of the HEK cells. Because HEK 293 is experimentally transformed, the cell line is not considered a good model direct cell research. But they are regularly used in experiments where the behavior of the cell itself is not being studied. HEK has been described as being a cellular test tube. The widespread use of this cell line is due to its extreme transfectability by the various techniques, including calcium phosphate method, achieving efficiencies approaching 100 percent The cell line is also used by the biotechnology industry to produce therapeutic proteins and viruses for gene therapy.

Altogen Research Services

Altogen Custom Services provide specialized biotechnology and pharmaceutical services, including - generation of stable cell lines, RNA Interference (RNAi) services, assay development, screening and transfection services. Generation of stably-expressing cancer cell lines and primary cells can be very expensive and time-consuming. Altogen Services offers generation of stable cell lines by transforming the cell line of choice to stably express gene of interest or shRNA-encoding construct. Cloning and shRNA expression services are available.