HeLa Transfection Reagent

Catalog # 2085 - 0.5 mL        $ 95.00 Catalog # 2086 - 1.5 mL        $ 230.00 Transfection reagent for HeLa cells Kit includes Transfection Enhancer reagent and recommended transfection protocols Protocols provided for transfection of DNA, mRNA, siRNA and microRNA High transfection efficiency in human cervix adenocarcinoma cells Suitable for co-transfection experiments High cell viability Compatible with transfection in any plate format Reproducible and reliable results Minimal off-target effects Optimized transfection protocols are adapted for use with both standard & reverse transfection methods Developed and manufactured by Altogen Biosystems Transfection Efficiency: Reagent exhibits at least 90% transfection efficiency of siRNA delivery. Transfection efficiency was determined by real-time RT-PCR. Transfection Protocol: Transfection protocols are provided with the purchase of the reagent.

DATA

Figure 1. Cyclophilin B silencing efficiency was determined by qRT-PCR in the cells transfected by Cyclophilin B siRNA or non-silencing siRNA control following the recommended transfection protocol. Cyclophilin mRNA expression levels were measured 48 hours post-transfection. 18S rRNA levels were used to normalize the Cyclophilin B data. Values are normalized to untreated sample. Data are presented as means ± SD (n=3).

siRNA Transfection

Gene silencing by RNA Interference (RNAi) is a powerful research tool for studying gene function in mammalian cells. RNAi is a biological phenomenon by which double stranded RNA (dsRNA) specifically reduces gene expression of its corresponding gene. Potent inhibition of specific gene expression is experimentally achieved by the transfection of small interfering RNA (siRNA), which binds RISC complex and cause degradation of target complementary mRNA molecules in the cell. Therefore, successful, potent RNAi experimentation is dependent upon the highly efficient delivery of the siRNA into cells by transfection of stable and functional siRNA molecules. Most DNA transfection reagents are incompatible with siRNA, sensitive to serum, or are capable of inducing cytotoxicity, which negatively affects gene expression studies. Transfection Reagents have been developed and optimized specifically for use with siRNA transfection and allow transfection in the presence of serum without lowering transfection efficiency or reducing cell viability. Ready-to-run transfection protocols eliminate the need for extensive siRNA optimization experiments. Altogen Biosystems kits are also compatible for transfection of microRNA (miRNA) molecules.

Human Cervix Adenocarcinoma Cells

One of the most famous cell lines, HeLa is named after Henrietta Lacks, a 31 year-old African-American women suffering from cervical cancer. The cells were derived from a tissue sample excised from the adenocarcinoma. HeLa were the first human cells able to survive indefinitely in vitro. HeLa cells have a modal chromosome number of 82, with four copies of chromosome 12 and three copies of chromosomes 6, 8, and 17. HeLa tests positive for keratin and lysophosphatidylcholine (lyso-PC). Hela cells are also known to contain human papilloma virus 18 sequences. There are now many strains of HeLa cells but all are descended from the same tumor cells removed from Lacks. HeLa cells proliferate abnormally fast because they have an active version of telomerase during cell division, which prevents aging and eventual cell death. HeLa cells were used by Jonas Salk to test the first polio vaccine in the 1950’s. They have also been used in studies researching cancer, AIDS, the effects of radiation and toxic substances and gene mapping.

Transfection Resource

siRNA Transfection || Transfection Methods || Stable Transfection || RNAi || siRNA Library Screening || RNAi Therapeutics || Cell Transfection || In Vivo Transfection Reagents || Cell Line Specific Transfection Reagents

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Altogen Research Services

Altogen Custom Services provide specialized biotechnology and pharmaceutical services, including - generation of stable cell lines, RNA Interference (RNAi) services, assay development, screening and transfection services. Generation of stably-expressing cancer cell lines and primary cells can be very expensive and time-consuming. Altogen Services offers generation of stable cell lines by transforming the cell line of choice to stably express gene of interest or shRNA-encoding construct. Cloning and shRNA expression services are available.