L929 Transfection Reagent

Catalog # 1785 - 0.5 mL        $ 120.00 Catalog # 1786 - 1.5 mL        $ 251.00 Transfection reagent for L929 cells Protocols provided for transfection of siRNA and microRNA Kit includes Transfection Enhancer reagent and recommended transfection protocols High cell viability Optimized for Murine Fbrosarcoma Cells Compatible with transfection in any plate format Reproducible transfection Optimized transfection protocols are adapted for use with both standard & reverse transfection methods Developed and manufactured by Altogen Biosystems Transfection Efficiency: Reagent exhibits at least 80% transfection efficiency of siRNA delivery. Transfection efficiency was determined by fluorescent microscopy and real-time RT-PCR. Transfection Protocol: Transfection protocols are provided with the purchase of the reagent.

Transfection Resource

Visit Altogen Biosystems Transfection Resource: RNAi applications (siRNA transfection, microRNA transfection, plasmid DNA transfection), stable and transient DNA transfection, delivery methods, electroporation, microinjection, dendrimer-based, non-viral and virus-mediated gene delivery, liposome and non-liposomal reagents. Transfection of cancer cell lines vs primary cultures. RNA Interference as a research tool for gene expression studies, gene silencing in neuronal cells, RNAi therapeutics and In Vivo Transfection Reagents from Altogen Biosystems.

L929 (Murine Fibrosarcoma Cells)

L929 is derived from mouse connective tissue fibroblast. The line is used to assay TNF-alpha and TNF-beta. Treating L929 with TNF initiates apoptosis and results in cell death. Some L929 subclones have been created that allows detection of TNF at concentrations of approximately 30 pmol/L. L929 cells are 10 times more sensitive to TNF mediated cytotoxicity in the presence of lithium chloride. L929 cells are deficient in known cell-cell adhesion molecules. These cells also secrete a neurotrophic factor closely related to the beta subunit of NGF. In addition, L929 have been found to secrete a factor that promotes the differentiation of porcine peripheral blood mononuclear cells into macrophages and monocytes. L929 cells secrete a chemotactic factor specific for monocytes as well as an uncharacterized factor that promotes the growth of a large variety of cell lines. L929 cells proliferate to a variety of growth factors, including IL2, IL3, G-CSF, GM-CSF, and M-CSF.

Transfection Resource

siRNA Transfection || Transfection Methods || Stable Transfection || RNAi || siRNA Library Screening || RNAi Therapeutics || Cell Transfection || In Vivo Transfection Reagents || Cell Line Specific Transfection Reagents

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Altogen Research Services

Altogen Custom Services provide specialized biotechnology and pharmaceutical services, including - generation of stable cell lines, RNA Interference (RNAi) services, assay development, screening and transfection services. Generation of stably-expressing cancer cell lines and primary cells can be very expensive and time-consuming. Altogen Services offers generation of stable cell lines by transforming the cell line of choice to stably express gene of interest or shRNA-encoding construct. Cloning and shRNA expression services are available.