LIPID In Vivo Transfection Reagent

Catalog #5010 LIPID In Vivo Transfection Reagent, 0.5 mL (10 injections) $405

Catalog #5011 LIPID In Vivo Transfection Reagent, 1.5 mL (30 injections) $895

LIPID-based In Vivo Transfection Kit

siRNA and plasmid DNA in vivo delivery reagent

Modes of administration:

  • Systemic intravenous (i.v.) injection
  • Direct intratumoral (i.t.) injection
  • Intraperitoneal (i.p.) injection

Altogen's LIPID In Vivo Transfection Reagent

  • Cationic lipid liposome-based reagent
  • Lipid conjugated complexes are stable in serum for 16h
  • Efficient delivery to the liver, pancreas, kidney, and certain tumor types via systemic administration
  • Efficient siRNA and plasmid DNA delivery via direct subcutaneous tumor injection (various tumor types)
  • Minimal toxicity
  • Functionally tested in mice
  • Applicable for plasmid DNA/siRNA co-injection
  • Download LIPID-based in vivo transfection protocol: [PDF] [Word] 
  • Developed and manufactured by Altogen Biosystems

DATA

Lipid In Vivo Mouse Rat Transfection Reagent

Figure 1. Systemic administration (i.v.) of Lipid-based In Vivo reagent conjugated with siRNA targeting Lamin A/C mRNA or non-silencing control siRNA following the recommended protocol. Tissues were collected and RNA isolated 48 hours after first injection. Samples were analyzed by qRT-PCR for Lamin A/C gene expression levels. Ribosomal RNA levels were used to normalize the Lamin A/C data. Data are means ± SD (n=6).

Lipid Tumor In Vivo Mouse Rat Transfection Reagent

Figure 2. Intratumoral administration (i.t.) of Lipid-based In Vivo reagent conjugated with siRNA targeting Lamin A/C mRNA or non-silencing control siRNA following the recommended protocol. Tissues were collected and RNA isolated 48 hours after first injection. Samples were analyzed by qRT-PCR for Lamin A/C gene expression levels. Ribosomal RNA levels were used to normalize the Lamin A/C data. Data are means ± SD (n=6).

 

All Altogen® In Vivo Transfection Kits supplied with ready-to-run transfection protocols that eliminate the need for extensive transfection optimization experiments. Read more about in vivo siRNA transfection, research methods and experimental protocols at Altogen's Transfection Resource.

 

Altogen Labs offers contract research studies, including in vivo testing services, xenograft animal models, teratoma formation, tissue targeted siRNA delivery, and in vivo toxicology CRO services.