MRC-5 Transfection Reagent

Catalog # 3205 - 0.5 mL        $ 125.00 Catalog # 3206 - 1.5 mL        $ 283.00 Transfection reagent for MRC-5 cells Kit includes Transfection Enhancer reagent and recommended transfection protocols Protocols provided for transfection of siRNA and microRNA High transfection efficiency Optimized for human Diploid Fibroblast Cells Compatible with transfection in any plate format Reproducible transfection Optimized transfection protocols are adapted for use with both standard & reverse transfection methods Developed and manufactured by Altogen Biosystems Transfection Efficiency: Reagent exhibits at least 80% transfection efficiency of siRNA delivery. Transfection efficiency was determined by real-time RT-PCR. Transfection Protocol: Transfection protocols are provided with the purchase of the reagent.

DATA

Figure 1. SiRNAs targeting Lamin A/C mRNA or non-silencing control siRNA were transfected following the recommended protocol. At 48 hours post-transfection the cells were analyzed by qRT-PCR for gene expression levels. 18S rRNA levels were used to normalize the Lamin A/C data. Values are normalized to untreated sample. Data are means ± SD (n=3).

MRC-5 (Human Diploid Fibroblast Cells)

MRC-5 was derived from the pulmonary tissue of an aborted 14-week-old male human fetus in 1966. This is a normal diploid human cell line with 46,XY karyotype. The modal chromosome number was 46, occurring in 70 percent of cells. The rate of polyploidy was 3.6 percent. The line exhibits adherent growth to glass and polymer culture dishes. MRC-5 are capable of 42 to 46 population doublings before senescence occurs. MRC-5 cells have been shown to be negative for reverse transcriptase and they supports the growth of a broad range of viruses, including adenoviruses; coxsackie a; cytomegalovirus; echovirus; herpes simplex virus; poliovirus; rhinovirus; respiratory syncytial virus; and varicella zoster virus. MRC-5 cells are frequently used in a wide array of applications, such as virology-related transfection experiments, cytotoxicity assessments, and the development of vaccines.

Transfection Resource

siRNA Transfection || Transfection Methods || Stable Transfection || RNAi || siRNA Library Screening || RNAi Therapeutics || Cell Transfection || In Vivo Transfection Reagents || Cell Line Specific Transfection Reagents

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Altogen Research Services

Altogen Custom Services provide specialized biotechnology and pharmaceutical services, including - generation of stable cell lines, RNA Interference (RNAi) services, assay development, screening and transfection services. Generation of stably-expressing cancer cell lines and primary cells can be very expensive and time-consuming. Altogen Services offers generation of stable cell lines by transforming the cell line of choice to stably express gene of interest or shRNA-encoding construct. Cloning and shRNA expression services are available.