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MRC-5 Transfection Reagent  

Catalog # 3205 - 0.5 mL        $ 125.00

Catalog # 3206 - 1.5 mL        $ 283.00

Transfection reagent for MRC-5 cells

  • Kit includes Transfection Enhancer reagent and recommended transfection protocols
  • Protocols provided for transfection of siRNA and microRNA
  • High transfection efficiency
  • Work in presence of serum
  • Compatible with transfection in any plate format
  • Reproducible transfection
  • Optimized transfection protocols are adapted for use with both standard & reverse transfection methods
  • Developed and manufactured by Altogen Biosystems
  • Transfection Efficiency:
    Reagent exhibits at least 80% transfection efficiency of siRNA delivery. Transfection efficiency was determined by real-time RT-PCR.

    Transfection Protocol:
    Transfection protocols are provided with the purchase of the reagent.

DATA

mrc5

Figure 1. SiRNAs targeting Lamin A/C mRNA or non-silencing control siRNA were transfected following the recommended protocol. At 48 hours post-transfection the cells were analyzed by qRT-PCR for gene expression levels. 18S rRNA levels were used to normalize the Lamin A/C data. Values are normalized to untreated sample. Data are means ± SD (n=3).

Transfection Resource

Visit Altogen Biosystems Transfection Resource: RNAi applications (siRNA transfection, microRNA transfection, plasmid DNA transfection), stable and transient DNA transfection, delivery methods, electroporation, microinjection, dendrimer-based, non-viral and virus-mediated gene delivery, liposome and non-liposomal reagents. Transfection of cancer cell lines vs primary cultures. RNA Interference as a research tool for gene expression studies, gene silencing in neuronal cells, RNAi therapeutics and In Vivo Transfection Reagents from Altogen Biosystems.

Transfection Resource

siRNA Transfection || Transfection Methods || Stable Transfection || RNAi || siRNA Library Screening || RNAi Therapeutics || Cell Transfection || In Vivo Transfection Reagents || Cell Line Specific Transfection Reagents

Featured Products

In Vivo Transfection Reagents || Fibroblast Cells Transfection Reagent || Astrocyte Cells Transfection Reagent || HEK-293 Transfection Reagent || MEF Transfection Reagent || HepG2 Transfection Reagent || CHO Reagent || MDA-MB Transfection Reagent || Cos7 Transfection Reagent || A549 Transfection Reagent || NIH3T3 Reagent || HUVEC Transfection Reagent || LNCaP Transfection Reagent || MCF-7 Reagent || PC-12 Reagent

Altogen Research Services

Altogen Custom Services provide specialized biotechnology and pharmaceutical services, including - generation of stable cell lines, RNA Interference (RNAi) services, assay development, screening and transfection services. Generation of stably-expressing cancer cell lines and primary cells can be very expensive and time-consuming. Altogen Services offers generation of stable cell lines by transforming the cell line of choice to stably express gene of interest or shRNA-encoding construct. Cloning and shRNA expression services are available.

 

 

 

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