MRC5 Transfection Reagent (Diploid Fibroblast Cells)
Transfection Reagent for MRC5 Cells (Human Diploid Fibroblast Cells)
A nanoparticle-based liposome formulation
Transfection protocols provided for transfection of proteins, DNA, mRNA, siRNA, shRNA and microRNA
Transfection Enhancer reagent provided with the kit
Produce higher level of recombinant protein expression with minimal disruption of normal cell function
Generate physiologically relevant data you can trust
Effective for plasmid DNA/siRNA co-transfection
Easy-to-use transfection protocol with reproducible results
Developed and manufactured by Altogen Biosystems
Reagent exhibits at least 80% transfection efficiency of siRNA delivery. Transfection efficiency was determined by qRT-PCR.
Transfection Protocol and MSDS:
Download Altogen Biosystems MRC-5 Transfection Protocol: [PDF]
Download MSDS: [PDF]
MRC5 Cell Line:
MRC-5 was derived from the pulmonary tissue of an aborted 14-week-old male human fetus in 1966. This is a normal diploid human cell line with 46,XY karyotype. The modal chromosome number was 46, occurring in 70 percent of cells. The rate of polyploidy was 3.6 percent. The line exhibits adherent growth to glass and polymer culture dishes. MRC-5 are capable of 42 to 46 population doublings before senescence occurs. MRC-5 cells have been shown to be negative for reverse transcriptase and they supports the growth of a broad range of viruses, including adenoviruses; coxsackie a; cytomegalovirus; echovirus; herpes simplex virus; poliovirus; rhinovirus; respiratory syncytial virus; and varicella zoster virus. MRC-5 cells are frequently used in a wide array of applications, such as virology-related transfection experiments, cytotoxicity assessments, and the development of vaccines. MRC-5 is useful as a transfection host, and in many kinds of molecular and cell biology research, in part due to its ability to be easily infected by viruses. This cell line is often used for testing of viruses in biomedical research experiments.
Figure 1. SiRNAs targeting Lamin A/C mRNA or non-silencing control siRNA were transfected into MRC5 following the recommended protocol. At 48 hours post-transfection the cells were analyzed by qRT-PCR for gene expression levels. 18S rRNA levels were used to normalize the Lamin A/C data. Values are normalized to untreated sample. Data are means ± SD (n=3).
Altogen Biosystems is a life sciences company that offers cell type-specific and pre-optimized transfection products, elecroporation kits, and in vivo delivery reagents (peg-liposome, nanoparticle, lipid, and polymer-based; and tissue targeted: liver, pancreas, kidney). Advanced formulation of reagents and optimized transfection protocols provide efficient intracellular delivery of protein, DNA, mRNA, shRNA and siRNA molecules. Read more about transfection technology at Altogen's Transfection Resource.
Altogen Research Services:
Altogen Labs is a GLP compliant laboratory that provides preclinical contract research services (CRO). Pharma and biotechnology services include: transient and stable transfection (development of stable cell lines), liposome encapsulation (proteins, DNA, mRNA, siRNA), A-to-Z RNAi services, in vivo and in vitro toxicology, xenograft animal models, assay development, cell banking and cryopreservation services.