Nanoparticle In Vivo Transfection Reagent

Catalog #5030 NANOPARTICLE In Vivo Transfection Reagent, 0.5 mL (10 injections) $445

Catalog #5031 NANOPARTICLE In Vivo Transfection Reagent, 1.5 mL (30 injections) $955

NANOPARTICLE-based In Vivo Transfection Kit

siRNA and plasmid DNA in vivo delivery reagent

Modes of administration:

  • Systemic intravenous (i.v.) injection
  • Direct intratumoral (i.t.) injection

Altogen's NANOPARTICLE In Vivo Transfection Reagent

  • Nanoparticle-based reagent
  • Complexes are stable in serum for 16h
  • Efficient delivery to the heart, lung, liver, pancreas, kidney, and multiple tumor types via systemic administration
  • Efficient siRNA and plasmid DNA delivery via direct subcutaneous tumor injection (various tumor types)
  • Minimal toxicity
  • Functionally tested in mice
  • Applicable for plasmid DNA/siRNA co-injection
  • Download NANOPARTICLE-based in vivo transfection protocol: [PDF] [Word] 
  • Developed and manufactured by Altogen Biosystems

DATA

In Vivo Transfection Reagent Mouse Rat Lung Liver Heart Brain Tumor

Figure 1. Systemic administration (i.v.) of Nanoparticle-based In Vivo reagent conjugated with siRNA targeting Lamin A/C mRNA or non-silencing control siRNA following the recommended protocol. Tissues were collected and RNA isolated 48 hours after first injection. Samples were analyzed by qRT-PCR for Lamin A/C gene expression levels. Ribosomal RNA levels were used to normalize the Lamin A/C data. Data are means ± SD (n=6).

Nanoparticle Invivo Transfection Reagent Mouse Rat

Figure 2. Intratumoral administration (i.t.) of Nanoparticle-based In Vivo reagent conjugated with siRNA targeting Lamin A/C mRNA or non-silencing control siRNA following the recommended protocol. Tissues were collected and RNA isolated 48 hours after first injection. Samples were analyzed by qRT-PCR for Lamin A/C gene expression levels. Ribosomal RNA levels were used to normalize the Lamin A/C data. Data are means ± SD (n=6).

 

All Altogen® In Vivo Transfection Kits supplied with ready-to-run transfection protocols that eliminate the need for extensive transfection optimization experiments. Read more about in vivo siRNA transfection, research methods and experimental protocols at Altogen's Transfection Resource.

 

Altogen Labs offers contract research studies, including in vivo testing services, xenograft animal models, teratoma formation, tissue targeted siRNA delivery, and in vivo toxicology CRO services.