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NIH3T3 Transfection Reagent |
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Catalog # 3211 - 0.5 mL $ 115.00
Catalog # 3212 - 1.5 mL $ 271.00
Transfection reagent for NIH3T3 cells
- Kit includes Transfection Enhancer reagent and recommended transfection protocols
- Protocols provided for transfection of mRNA, siRNA and microRNA
- High transfection efficiency
- Work in presence of serum
- Compatible with transfection in any plate format
- Reproducible transfection
- High cell viability
- Optimized transfection protocols are adapted for use with both standard & reverse transfection methods
- Developed and manufactured by Altogen Biosystems
Transfection Efficiency:
Reagent exhibits at least 85% transfection
efficiency of siRNA delivery. Transfection efficiency
was determined by real-time RT-PCR.
Transfection Protocol:
Transfection protocols are provided with
the purchase of the reagent.
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DATA |
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Figure 1. GAPD mRNA levels were quantified using real-time RT-PCR in the cells transfected with siRNAs targeting GAPD or non-silencing siRNA. Forty-eight hours post-transfection, the cells were harvested and analyzed by real-time RT-PCR for GAPDH mRNA expression levels. Data were normalized against the 18S rRNA signal. Control samples were either mock-transfected or untreated. Values are normalized to untreated sample. Data are means ± SD (n=3).
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Transfection Resource |
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siRNA Transfection || Transfection Methods || Stable Transfection || RNAi || siRNA Library Screening || RNAi Therapeutics || Cell Transfection || In Vivo Transfection Reagents || Cell Line Specific Transfection Reagents
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In Vivo Transfection Reagents || Fibroblast Cells Transfection Reagent || Astrocyte Cells Transfection Reagent || HEK-293 Transfection Reagent || MEF Transfection Reagent || HepG2 Transfection Reagent || CHO Reagent || MDA-MB Transfection Reagent || Cos7 Transfection Reagent || A549 Transfection Reagent || NIH3T3 Reagent || HUVEC Transfection Reagent || LNCaP Transfection Reagent || MCF-7 Reagent || PC-12 Reagent
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Altogen Research Services |
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Altogen Custom Services provide specialized biotechnology and pharmaceutical services, including - generation of stable cell lines, RNA Interference (RNAi) services, assay development, screening and transfection services. Generation of stably-expressing cancer cell lines and primary cells can be very expensive and time-consuming. Altogen Services offers generation of stable cell lines by transforming the cell line of choice to stably express gene of interest or shRNA-encoding construct. Cloning and shRNA expression services are available.
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