NIH3T3 Transfection Reagent

Catalog # 3211 - 0.5 mL        $ 115.00 Catalog # 3212 - 1.5 mL        $ 271.00 Transfection reagent for NIH3T3 cells Kit includes Transfection Enhancer reagent and recommended transfection protocols Protocols provided for transfection of mRNA, siRNA and microRNA Optimized for Murine Embryonic Fibroblast Cells Work in presence of serum Compatible with transfection in any plate format Reproducible transfection High cell viability Optimized transfection protocols are adapted for use with both standard & reverse transfection methods Developed and manufactured by Altogen Biosystems Transfection Efficiency: Reagent exhibits at least 85% transfection efficiency of siRNA delivery. Transfection efficiency was determined by real-time RT-PCR. Transfection Protocol: Transfection protocols are provided with the purchase of the reagent.

DATA

Figure 1. GAPD mRNA levels were quantified using real-time RT-PCR in the cells transfected with siRNAs targeting GAPD or non-silencing siRNA. Forty-eight hours post-transfection, the cells were harvested and analyzed by real-time RT-PCR for GAPDH mRNA expression levels. Data were normalized against the 18S rRNA signal. Control samples were either mock-transfected or untreated. Values are normalized to untreated sample. Data are means ± SD (n=3).

Murine Embryonic Fibroblast Cells

NIH3T3 are derived from a cell line of from Swiss mouse embryo tissue established in 1962 by two scientists at the Department of Pathology in the New York University School of Medicine. The 3T3 cell line has become the standard fibroblast cell line. Mouse embryonic fibroblasts NIH3T3 is a type of murine embryonic fibroblast cells. MEFs are often used as "feeder cells" in human embryonic stem cell research and for the maintenance of embryonic stem cells in the undifferentiated state. MEF are also used for titration of viruses from mouse organs and tissues in lab studies. NIH3T3 cells are often used in the cultivation of keratinocytes, with the 3T3 cells secreting growth factors favorable to keratinocytes. It has been found that NIH3T3 cells are inhibited by benzodiazepines. The 3T3 designation is an abbreviation of “3-day transfer, inoculum 3 x 10e5 cells“.

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Altogen Research Services

Altogen Custom Services provide specialized biotechnology and pharmaceutical services, including - generation of stable cell lines, RNA Interference (RNAi) services, assay development, screening and transfection services. Generation of stably-expressing cancer cell lines and primary cells can be very expensive and time-consuming. Altogen Services offers generation of stable cell lines by transforming the cell line of choice to stably express gene of interest or shRNA-encoding construct. Cloning and shRNA expression services are available.