PAE Transfection Reagent

Catalog # 3223 - 0.5 mL        $ 98.00 Catalog # 3224 - 1.5 mL        $ 241.00 Transfection reagent for PAE cells Protocols provided for transfection of mRNA, DNA, siRNA and microRNA Kit includes Transfection Enhancer reagent and recommended transfection protocols High transfection efficiency Low toxicity Compatible with transfection in any plate format Reproducible transfection Cost-effective transfection Developed and manufactured by Altogen Biosystems Transfection Efficiency: Reagent exhibits at least 85% transfection efficiency of siRNA delivery. Transfection efficiency was determined by real-time RT-PCR. Transfection Protocol: Transfection protocols are provided with the purchase of the reagent.

Transfection Resource

Visit Altogen Biosystems Transfection Resource: RNAi applications (siRNA transfection, microRNA transfection, plasmid DNA transfection), stable and transient DNA transfection, delivery methods, electroporation, microinjection, dendrimer-based, non-viral and virus-mediated gene delivery, liposome and non-liposomal reagents. Transfection of cancer cell lines vs primary cultures. RNA Interference as a research tool for gene expression studies, gene silencing in neuronal cells, RNAi therapeutics and In Vivo Transfection Reagents from Altogen Biosystems.

Altogen Biosystems

Altogen Biosystems is a life sciences company dedicated to the development, marketing and manufacture of cell type specific transfection reagents. Efficient delivery of DNA and RNA molecules (siRNA, plasmid DNA, mRNA, microRNA) enabled by advanced formulation of reagents and peculiar design of transfection protocols. Altogen Biosystems offers a complete transfection system for a broad range of cell lines and primary cells. Reagents are functionally tested to be reproducible, serum compatible, induce low toxicity, and can be used for co-transfection experiments, and high throughput applications.

Transfection Resource

siRNA Transfection || Transfection Methods || Stable Transfection || RNAi || siRNA Library Screening || RNAi Therapeutics || Cell Transfection || In Vivo Transfection Reagents || Cell Line Specific Transfection Reagents

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Porcine Aortic Endothelial Cells)

Porcine aortic endothelial cells (PAEC) are derived from healthy, plaque-free porcine aorta. PAOEC can be cultured and propagated at least 16 population doublings. Arterial endothelial cells play a vital role cardiac homeostasis. Insults to the endothelial cells have been linked to a variety of vascular diseases. Porcine artery endothelial cells have been used as a practical alternative for the study of artery endothelial function and metabolism, including atherosclerosis, relaxing factor synthesis, and antioxidant enzyme activities.PAOEC produce glycoproteins with important biological functions, including the control of cell adhesion, blood clotting, blood pressure, the immune system, and apoptosis. Species-specific monoclonal antibodies suggest that PAECs directly present swine MHC antigens to human T cells. Additionally, PAECs bind CTLA-4-Ig and costimulate human T cells by the CD2 and CD28 pathways. Studies have shown that primary cultures of porcine aortic endothelial cells express connexin 37 and 43, and provide strong evidence that the second messenger molecule InsP3 can permeate porcine endothelial gap junctions.