PC-12 Transfection Reagent

Catalog # 3229 - 0.5 mL        $ 111.00 Catalog # 3230 - 1.5 mL        $ 220.00 Transfection reagent for PC-12 cells Protocols provided for transfection of siRNA and microRNA High transfection efficiency Optimized for PC-12 (Rat Pheochromocytoma Cells) Kit includes Transfection Enhancer reagent and recommended transfection protocols Compatible with transfection in any plate format Reproducible transfection Optimized transfection protocols are adapted for use with both standard & reverse transfection methods Developed and manufactured by Altogen Biosystems Transfection Efficiency: Reagent exhibits at least 80% transfection efficiency of siRNA delivery. Transfection efficiency was determined by real-time RT-PCR. Transfection Protocol: Transfection protocols are provided with the purchase of the reagent.

DATA

Figure 1. SiRNAs targeting Lamin A/C mRNA or non-silencing control siRNA were transfected following the recommended protocol. At 48 hours post-transfection the cells were analyzed by qRT-PCR for gene expression levels. 18S rRNA levels were used to normalize the Lamin A/C data. Values are normalized to untreated sample. Data are means ± SD (n=3).

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PC-12 (Rat Pheochromocytoma Cells)

PC-12 was originally derived in 1976 from a transplantable rat adrenal pheochromocytoma in 1976. PC-12 are small, have a limited amount of cytoplasm, are difficult to transfect, and have a doubling time of more than two days. These cells stop dividing and terminally differentiate when treated with nerve growth factor, which makes PC-12 cells useful as a model system for neuronal differentiation. PC-12 treated with nerve growth factor stop proliferation, grow long neuritis, and exhibit cellular composition changes that are associated with neuronal differentiation. When PC-12 differentiate, they are even more difficult to transfect. Both neuronal NO synthase and nitric oxide are required for PC12 cell differentiation. Research suggests PC-12 cells may represent a stage specific vulnerability to a known neurotoxic agent. PC-12 have a rounded morphology, tend to form clusters in culture and attach poorly to non-coated plastic surfaces, making them difficult to culture.