PC-3 Transfection Reagent

Catalog # 3227 - 0.5 mL        $ 110.00 Catalog # 3228 - 1.5 mL        $ 220.00 Transfection reagent for PC-3 cells Protocols provided for transfection of mRNA, siRNA and microRNA Kit includes Transfection Enhancer reagent and recommended transfection protocols Optimized for PC-3 (Human Prostate Cancer Cells) Compatible with transfection in any plate format Reproducible transfection Minimal off-target effects Optimized transfection protocols are adapted for use with both standard & reverse transfection methods Developed and manufactured by Altogen Biosystems Transfection Efficiency: Reagent exhibits at least 70% transfection efficiency of siRNA delivery. Transfection efficiency was determined by real-time RT-PCR. Transfection Protocol: Transfection protocols are provided with the purchase of the reagent.

Transfection Resource

siRNA Transfection || Transfection Methods || Stable Transfection || RNAi || siRNA Library Screening || RNAi Therapeutics || Cell Transfection || In Vivo Transfection Reagents || Cell Line Specific Transfection Reagents

PC-3 (Human Prostate Cancer Cells)

Considered a classical cell line of prostatic cancer, PC-3 was derived from the bone marrow metastasis isolated post-mortem from a 62-year-old Caucasian man with grade IV prostate cancer after androgen suppression therapy. PC-3 cells have high metastatic potential. PC-3 cells have been shown to form tumors in nude mice, grow in soft agar, be unresponsive to androgen treatment. PC-3 have low testosterone-5-alpha reductase activity and express Prostate Specific Antigen. This cell line has a modal number of 62 chromosomes. There are nearly 20 marker chromosomes commonly found in each cell; and normal N2, N3, N4, N5, N12, and N15 are not found. No normal Y chromosomes could be detected by Q-band analysis. PC-3 cells express several cytokines and growth factors including vascular endothelial growth factors, fibroblast growth factors, interleukins and neurotrophic factors. It is suggested PC-3 cells release substances that influence the behavior of the cells in the tumor's microenvironment.

Altogen Research Services

Altogen Custom Services provide specialized biotechnology and pharmaceutical services, including - generation of stable cell lines, RNA Interference (RNAi) services, assay development, screening and transfection services. Generation of stably-expressing cancer cell lines and primary cells can be very expensive and time-consuming. Altogen Services offers generation of stable cell lines by transforming the cell line of choice to stably express gene of interest or shRNA-encoding construct. Cloning and shRNA expression services are available.