RAW-1 Transfection Reagent

Catalog # 3245 - 0.5 mL        $ 91.00 Catalog # 3246 - 1.5 mL        $ 220.00 Transfection reagent for RAW-1 cells Protocols provided for transfection of siRNA and microRNA High transfection efficiency Optimized for RAW RAW-1 and RAW-264.7 (Murine Macrophage Cells) Work in presence of serum Compatible with transfection in any plate format Reproducible transfection Kit includes Transfection Enhancer reagent and recommended transfection protocols Optimized transfection protocols are adapted for use with both standard & reverse transfection methods Developed and manufactured by Altogen Biosystems Transfection Efficiency: Reagent exhibits at least 75% transfection efficiency of siRNA delivery. Transfection efficiency was determined by real-time RT-PCR. Transfection Protocol: Transfection protocols are provided with the purchase of the reagent.

Transfection Resource

Visit Altogen Biosystems Transfection Resource: RNAi applications (siRNA transfection, microRNA transfection, plasmid DNA transfection), stable and transient DNA transfection, delivery methods, electroporation, microinjection, dendrimer-based, non-viral and virus-mediated gene delivery, liposome and non-liposomal reagents. Transfection of cancer cell lines vs primary cultures. RNA Interference as a research tool for gene expression studies, gene silencing in neuronal cells, RNAi therapeutics and In Vivo Transfection Reagents from Altogen Biosystems.

RAW-264.7 (Murine Macrophage Cells)

RAW 264.7 is a macrophage-like cell line derived from tumors induced in male BALB/c mice by the Abelson murine leukemia virus. The cells are negative for surface immunoglobulin and Thy-1.2. RAW264.7 does not secrete detectable virus particles and is negative in the XC plaque formation assay. These cells will pinocytose neutral red and will phagocytose latex beads and zymosan. The cells are used in metabolic, inflammation and apoptosis studies. They are capable of antibody dependent lysis of sheep erythrocytes and tumor cell targets. RAW 264.7 cells also express polytropic MuLV. The RAW 264.7 cells adhere to tissue culture-grade plastic through cation–dependent integrin receptors and other cation-independent receptors. They are extremely sensitive to lipopolysaccharide (LPS) endotoxin from gram-negative bacteria, therefore, only sterile disposable tissue culture ware and solutions, buffers, and media with endotoxin tested distilled deionized water should be used.

Transfection Resource

siRNA Transfection || Transfection Methods || Stable Transfection || RNAi || siRNA Library Screening || RNAi Therapeutics || Cell Transfection || In Vivo Transfection Reagents || Cell Line Specific Transfection Reagents

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In Vivo Transfection Reagents || Fibroblast Cells Transfection Reagent || Astrocyte Cells Transfection Reagent || HEK-293 Transfection Reagent || MEF Transfection Reagent || HepG2 Transfection Reagent || CHO Reagent || MDA-MB Transfection Reagent || Cos7 Transfection Reagent || A549 Transfection Reagent || NIH3T3 Reagent || HUVEC Transfection Reagent || LNCaP Transfection Reagent || MCF-7 Reagent || PC-12 Reagent

Altogen Research Services

Altogen Custom Services provide specialized biotechnology and pharmaceutical services, including - generation of stable cell lines, RNA Interference (RNAi) services, assay development, screening and transfection services. Generation of stably-expressing cancer cell lines and primary cells can be very expensive and time-consuming. Altogen Services offers generation of stable cell lines by transforming the cell line of choice to stably express gene of interest or shRNA-encoding construct. Cloning and shRNA expression services are available.