WERI-Rb-1 Transfection Reagent

Catalog # 3247 - 0.5 mL        $ 87.00 Catalog # 3248 - 1.5 mL        $ 205.00 Transfection reagent for WERI-Rb-1 cells Protocols provided for transfection of DNA, siRNA and microRNA Optimized for WERI-Rb-1 (Retinoblastoma Cells) Work in presence of serum Compatible with transfection in any plate format Reproducible transfection Kit includes Transfection Enhancer reagent and recommended transfection protocols Optimized transfection protocols are adapted for use with both standard & reverse transfection methods Developed and manufactured by Altogen Biosystems Transfection Efficiency: Reagent exhibits at least 75% transfection efficiency of siRNA delivery. Transfection efficiency was determined by real-time RT-PCR. Transfection Protocol: Transfection protocols are provided with the purchase of the reagent.

WERI-Rb-1 (Retinoblastoma Cells)

WERI-Rb-1 was derived in 1974 from retinoblastoma tissue of a 1-year-old Caucasian girl in 1974. This is a near diploid line. The modal chromosome number is 47 occurring at 38 percent and the rate of polyploidy is nine percent. Fifteen to sixteen marker chromosomes are present in all cells. Normal chromosomes 3, 10, 13 and 16 are absent. There are two copies of the X chromosome. No Y chromosomes were detected in QM stained preparations. The cells survive culturing in Difco Bacto-Agar but do not form colonies. The cells grow in grape-like clusters of round cells in suspension. Scanning electron microscopy reveals some variation in the number and frequency of surface blebs, lamellipodia and microvilli. The line is of interest in studies of cell differentiation, animal models of tumor therapy and biochemical evaluations.

Altogen Biosystems

Altogen Biosystems is a life sciences company dedicated to the development, marketing and manufacture of cell type specific transfection reagents. Efficient delivery of DNA and RNA molecules (siRNA, plasmid DNA, mRNA, microRNA) enabled by advanced formulation of reagents and peculiar design of transfection protocols. Altogen Biosystems offers a complete transfection system for a broad range of cell lines and primary cells. Reagents are functionally tested to be reproducible, serum compatible, induce low toxicity, and can be used for co-transfection experiments, and high throughput applications.

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Altogen Research Services

Altogen Custom Services provide specialized biotechnology and pharmaceutical services, including - generation of stable cell lines, RNA Interference (RNAi) services, assay development, screening and transfection services. Generation of stably-expressing cancer cell lines and primary cells can be very expensive and time-consuming. Altogen Services offers generation of stable cell lines by transforming the cell line of choice to stably express gene of interest or shRNA-encoding construct. Cloning and shRNA expression services are available.