SK-N-MC Transfection Reagent

Catalog # 3261 - 0.5 mL        $ 127.00 Catalog # 3262 - 1.5 mL        $ 275.00 Transfection reagent for SK-N-MC cells Protocols provided for transfection of siRNA and microRNA High transfection efficiency Optimized for SK-N-MC (Neuroblastoma Cells) Work in presence of serum Compatible with transfection in any plate format Reproducible transfection Kit includes Transfection Enhancer reagent and recommended transfection protocols Optimized transfection protocols are adapted for use with both standard & reverse transfection methods Developed and manufactured by Altogen Biosystems Transfection Efficiency: Reagent exhibits at least 85% transfection efficiency of siRNA delivery. Transfection efficiency was determined by real-time RT-PCR. Transfection Protocol: Transfection protocols are provided with the purchase of the reagent.

Transfection Resource

siRNA Transfection || Transfection Methods || Stable Transfection || RNAi || siRNA Library Screening || Cell Line Specific Transfection Reagents

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SK-N-MC (Neuroblastoma Cells)

SK-N-MC was derived in 1971 from a 14-year-old girl. The original paper indicated it came from the supraorbital metastasis of a neuroblastoma. However, SK-N-MC is now thought by many to have originated from an Askin’s tumor related to Ewing’s sarcoma, which is the morphologically similar to neuroblastoma. Research showed FLI1-EWS gene fusion, the molecular pathological change in Ewing’s sarcoma and Askin’s tumor, in the cells. SK-N-MC are adherent, fibroblast-like cells growing as monolayers. The cell line is a pseudodiploid human female (XX), with chromosome counts in the diploid range. SK-N-MC has a modal chromosome number of 46. Normal chromosomes N3 and N10 are absent, and many are monosomic. Normal chromosome N8 is most often tetrasomic. The remainder of normal chromosomes were usually paired. It has moderate dopamine-beta-hydroxylase activity as well as formaldehyde induced fluorescence indicative of intracellular catecholamines.

SH-SY5Y is a triple-cloned neuroblastoma which originated in 1978 from the SK-N-SH line. A neuroblast -like subclone of SK-N-SH, named SH-SY, was subcloned as SH-SY5, which was subcloned again as SH-SY5Y. This cell line is genetically female, with two X chromosomes. The original SK-N-SH line was derived in 1970 from a four year old girl during a bone marrow biopsy of a metastatic neuroblastoma. SH-SY5Y possess an abnormal chromosome called trisomy 1q. The cell line has been shown to be dopamine beta hydroxylase active, acetylcholinergic, glutamatergic and adenosinergic. SH-SY5Y propagate through mitosis and differentiate by extending neurites to the surrounding area. The dividing cells can form clusters of cells which are reminders of their cancerous nature, but certain treatments such as retinoic acid and BDNF can force the cells to dendrify and differentiate. It has been shown that citrus limonoid glucosides are toxic to SH-SY5Y cancer cells.