Transfection Reagents

Transfection by calcium phosphate was one of the earliest chemical based methods developed to deliver exogenous nucleic acids to cultured mammalian cells. This method is dependent upon the use of a calcium chloride-containing HEPES-buffered saline solution to deliver plasmid DNA. Advances in the development of lipid and polymer-based carrier molecules capable of binding nucleic acids led to the adaptation of these compounds for transfection. Many of these compounds create liposomes, which can fuse with the cellular membrane in order to deliver the bound RNA or DNA to the cell. Recently, non-liposomal lipids and organic compounds called dendrimers have been developed and applied as a transfection agent for use with difficult to transfect cell lines. The in vitro uses of these methods are a powerful tool in basic life sciences research and associated clinical studies.

ALTOGEN® Transfection Reagents:

In Vivo Transfection Reagent (NANOPARTICLE-based)

In Vivo Transfection Reagent (LIPID-based)

In Vivo Transfection Reagent (POLYMER-based)

3LL Transfection Reagent (murine Lewis lung carcinoma cells)

A204 Transfection Reagent (human rhabdomyosarcoma cells)

A549 Transfection Reagent (human lung carcinoma cells)

ASMC Transfection Reagent (aortic smooth muscle cells)

AsPC-1 Transfection Reagent (human pancreatic cells)

Astrocytes Transfection Reagent (primary astrocyte cells)

BMS2 Transfection Reagent (bone marrow stromal cells)

Caki-1 Transfection Reagent (renal carcinoma cells)

Calu-6 Transfection Reagent (human renin-expressing cells)

CFPEo Transfection Reagent (human trachea epithelium cells)

CHO Transfection Reagent (hamster ovary epithelial cells)

Chromaffin cells Transfection Reagent (chromaffin cell cultures)

CLBPEC Transfection Reagent (human neuroblastoma cells)

Cos-7 Transfection Reagent (monkey kidney cells)

D-407 Transfection Reagent (retinal pigment epithelial cells)

D-451 Transfection Reagent (murine fibroblast cells)

DI-TNC1 Transfection Reagent (rat brain astrocyte cells)

DU145 Transfection Reagent (human prostate cancer cells)

FaDu Transfection Reagent (human hypopharyngeal carcinoma cells)

Fibroblasts Transfection Reagent (primary fibroblast cells)

HCAEC Transfection Reagent (human coronary artery endothelial cells)

HCN-1A Transfection Reagent (human cortical neuronal cells)

HCS-2 Transfection Reagent (human chondrosarcoma cells)

HEK-293 Transfection Reagent (human epithelial kidney cells)

HeLa Transfection Reagent (human cervix adenocarcinoma cells)

HepG2 Transfection Reagent (human hepatocellular carcinoma cells)

HMEC Transfection Reagent (human mammary epithelial cancer cells)

HT-29 Transfection Reagent (human colorectal adenocarcinoma cells)

HTB Transfection Reagent (human osteosarcoma cells)

HuH-7 Transfection Reagent (human hepatoma cells)

HUVEC Transfection Reagent (umbilical vein endothelial cells)

KB Transfection Reagent (human mouth epidermal carcinoma cells)

Keratinocyte Transfection Reagent (keratinocyte cells)

L929 Transfection Reagent (murine fibrosarcoma cells)

LNCaP Transfection Reagent (prostate cancer cells)

MA-10 Transfection Reagent (Leydig tumour cells)

MCF-7 Transfection Reagent (human breast cancer cells)

MDA-MB Transfection Reagent (breast cancer cells)

MEF Transfection Reagent (murine embryonic fibroblast cells)

MES Transfection Reagent (mouse embryonic stem cells)

MM39 Transfection Reagent (human tracheal gland cells)

MRC-5 Transfection Reagent (human diploid fibroblast cells)

NIH3T3 Transfection Reagent (mouse fibroblast cells)

NRK Transfection Reagent (normal rat kidney epithelial cells)

P815 Transfection Reagent (murine mastocytoma cells)

PAE Transfection Reagent (porcine aortic endothelial cells)

PANC Transfection Reagent (human pancreatic cancer cells)

PC-3 Transfection Reagent (human prostate cancer cells)

PC-12 Transfection Reagent (rat pheochromocytoma cells)

PGHAM Transfection Reagent (hamster pancreatic carcinoma cells)

PHC Transfection Reagent (primary hepatocarcinoma cells)

PS-1 Transfection Reagent (prostate smooth muscle cells)

RAW-1 Transfection Reagent (murine macrophage cells)

SaOS Transfection Reagent (human osteoblastic cells)

SHEP Transfection Reagent (human neuroblastoma cells)

SKNAS Transfection Reagent (neuroblastoma cells)

SK-N-MC Transfection Reagent (neuroblastoma cells)

SW-13 Transfection Reagent (human adrenocortical carcinoma cells)

U87 Transfection Reagent (glioblastoma cells)

VERO Transfection Reagent (African green monkey kidney cells)

WERI-Rb-1 Transfection Reagent (retinoblastoma cells)

Transfection Resource

siRNA Transfection | Transfection Methods | Stable Transfection | RNAi | siRNA Library Screening | RNAi Therapeutics | Cell Transfection | In Vivo Transfection Reagents | Cell Line Specific Transfection Reagent

Altogen Biosystems

Altogen Biosystems is a life sciences company dedicated to the development, marketing and manufacture of cell type specific transfection reagents. Efficient delivery of DNA, RNA, siRNA, and microRNA enabled by advanced formulation of reagents and peculiar design of protocols.

Altogen Biosystems offers a complete transfection system for a broad range of cell lines. All reagents are functionally tested to be highly reproducible, serum compatible, induce low toxicity, and can be used for co-transfection experiments, and high throughput applications.