U87 Transfection Reagent

Catalog # 3277 - 0.5 mL        $ 149.00 Catalog # 3278 - 1.5 mL        $ 223.00 Transfection reagent for U87 cells Protocols provided for transfection of DNA, mRNA, siRNA and microRNA High transfection efficiency Kit includes Transfection Enhancer reagent and recommended transfection protocols Optimized for U87 and U87MG (Glioblastoma Cells) Reproducible transfection Optimized transfection protocols are adapted for use with both standard & reverse transfection methods Developed and manufactured by Altogen Biosystems Transfection Efficiency: Reagent exhibits at least 85% transfection efficiency of siRNA delivery. Transfection efficiency was determined by real-time RT-PCR. Transfection Protocol: Transfection protocols are provided with the purchase of the reagent.

Transfection Resource

Visit Altogen Biosystems Transfection Resource: RNAi applications (siRNA transfection, microRNA transfection, plasmid DNA transfection), stable and transient DNA transfection, delivery methods, electroporation, microinjection, dendrimer-based, non-viral and virus-mediated gene delivery, liposome and non-liposomal reagents. Transfection of cancer cell lines vs primary cultures. RNA Interference as a research tool for gene expression studies, gene silencing in neuronal cells, RNAi therapeutics and In Vivo Transfection Reagents from Altogen Biosystems.

U87MG (Glioblastoma Cells)

U-87 MG is a human glioblastoma cell line derived from a stage three 44 year-old Caucasian female. It has epithelial morphology. Grade IV glioma, also called glioblastoma multiforme (GBM), is the most common primary malignant brain tumor with about 16,000 new diagnoses each year in the United States. GBMs have a one-year survival rate of only 29.6 percent making it one of the most deadly types of cancer. This is a hypodiploid human cell line with the modal chromosome number of 44 occurring in 48 percent of cells. The rate of higher ploidy was 5.9 percent. Twelve markers were common to all cells. There was only one copy of normal X. N1, N6 and N9 were not found. U-87 MG cells are used as an vitro model of human glioblastoma cells to investigate the cytotoxic effect of chemotherapeutic drugs towards cancer cells.

Transfection Resource

siRNA Transfection | Transfection Methods | Stable Transfection | RNAi | siRNA Library Screening | RNAi Therapeutics | Cell Transfection | In Vivo Transfection Reagents | Cell Line Specific Transfection Reagents

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Altogen Research Services

Altogen Custom Services provide specialized biotechnology and pharmaceutical services, including - generation of stable cell lines, RNA Interference (RNAi) services, assay development, screening and transfection services. Generation of stably-expressing cancer cell lines and primary cells can be very expensive and time-consuming. Altogen Services offers generation of stable cell lines by transforming the cell line of choice to stably express gene of interest or shRNA-encoding construct. Cloning and shRNA expression services are available.